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Laboratory 2 > Serologic Testing > Flashcards

Serologic Testing Flashcards

1
Q

What is Rapid Plasma Reagin?

A

Non-treponemal test for serologic detecting of the antibody Reagin

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2
Q

Why is the antibody Reagin produced?

A

Produced in response to an infection of syphilis caused by Treponema pallidum

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3
Q

What is the RPR reagin?

A

Phospholipid and is found in the serum/plasma of patients with syphilis

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4
Q

What is the causative agent of syphilis?

A

Treponema pallidum

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5
Q

What is the vehicle or “antigen” used to see the macroscopic results of RPR?

A

Carbon charcoal particle

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6
Q

Does the RPR test require confirmation?

A

Yes, it is only a screening test

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7
Q

What is a positive RPR test?

A

If flocculation is present

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8
Q

What is the confirmatory test for RPR?

A

Fluorescent treponemal antibody-absorbed test (FTA-ABS)

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9
Q

What will be shown on a confirmatory FTA-ABS test?

A

Slide will fluoresce under UV light on a positive test

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10
Q

What two tubes of blood will collect for serum and plasma for RPR test?

A
  • Serum: Red/SST (without anticoagulation)

- Plasma: Purple ( with anticoagulation)

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11
Q

How should the serum look after centrifuge for RPR?

A

Clear and non-hemolyzed

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12
Q

What is included within the RPR test kit?

A
  • RPR Card antigen suspension
  • Plastic disposable bottle
  • 20 gauge, galvanized needle, blunt tip
  • Test cards
  • Pipettes/stirrers
  • RPR Test control cards
  • 1 mL Tuberculin syringe
  • Distilled water
  • Mechanical rotator
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13
Q

What need to be done prior to RPR testing?

A
  • Centrifuge specimen
  • Mix antigen by shaking vigorously for 10-15 seconds
  • Attach needle to hub of dispensing bottle
  • Snap off top of ampule and draw antigen into dispensing bottle
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14
Q

How many drops do you transfer from specimen to card for RPR testing?

A

One drop

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15
Q

How many drops of antigen to the specimen and control to be tested with dispensing bottle?

A

One drop

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16
Q

How long do you place test card and controls on the rotator and at what rpm?

A
  • 8 minutes

- 100 prm

17
Q

When do you read the test card for RPR?

A

At the end of 8 minutes

18
Q

How many times do you rotate and tilt the RPR card?

A

3-4 times

- use a to-fro motion

19
Q

How do you read the RPR card?

A

Macroscopically

20
Q

What is the range of flocculation in a reactive test?

A
  • Slight but definite
  • Minimum to moderate
  • Marked and intense
21
Q

How is RPR reported?

A

Either reactive or non-reactive

22
Q

What are the two QC sections of serology testing?

A
  • Equipment QC

- Reagent QC

23
Q

Regarding equipment QC, what does the mechanical rotator need to be calibrated to?

A

100 RPM

24
Q

Regarding equipment QC, what does the antigen needle need to be calibrated to?

A

60 drops/mL

25
Q

What are the two pieces of equipment within equipment QC?

A
  • Mechanical rotator

- Antigen needle

26
Q

What are the three controls for Reagent QC in RPR testing?

A
  • Reactive
  • Weak-reactive
  • Non-reactive
27
Q

How are Control reagents treated in RPR testing?

A

Treated as Pt’s

28
Q

What is the monospot test used to detect?

A

Infectious mononucleosis which is a self-limiting disease caused by Epstein-Barr virus

29
Q

When is infectious mononucleosis typically seen?

A

Young adults and children less than 5 yrs

30
Q

What is the presentation of infectious mononucleosis?

A
  • Fever
  • Malaise
  • Lethargy
  • Sore throat with exudates
  • Enlarged lymph nodes on the neck
  • Mild hepatitis
  • Enlarged spleen
  • Occasional blotchy skin rash
31
Q

What does infectious mononucleosis cause?

A

Accumulation of increased numbers and abnormal forms of lymphocytes and monocytes within lymph nodes
- Causes the formation of “heterophile” antibody

32
Q

What tubes do you collect blood in for infectious mononucleosis?

A

Tubes with or without anticoagulants

33
Q

What are the parts of the Monospot Latex Test Kit?

A
  • Latex Reagent suspension
  • Pipettes/stirrers
  • Test slides
  • Positive/negative control
34
Q

What should be done prior to conducting monospot testing?

A
  • Centrifuge the specimen
  • Test the specimen within 24 hours if stored between 2-8 degrees Celsius
  • Perform positive/negative QC
35
Q

To what temperature does the Latex reagent need to reach after refrigeration?

A

Room temperature (20-30 degrees Celsius)

36
Q

How long do you gently rotate the slide for when testing for infectious mononucleosis?

A
  • 3 minutes manually or on a rotary shaker set to 60-100 rpm
37
Q

How is infectious mononucleosis reported?

A

Positive or negative

38
Q

What does a positive test for infectious mononucleosis show?

A

Shows agglutination

39
Q

What does a negative test for infectious mononucleosis show?

A

Demonstrate a smooth, homogenous solution with no agglutination

Laboratory 2 (4 decks)

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