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Urinalysis & Body Fluids > Seminal Fluid Analysis > Flashcards

Seminal Fluid Analysis Flashcards

1
Q

purpose for seminal fluid analysis

A

evaluate infertility

post vasectomy

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2
Q

cells that produce sperm

A

Sertoli cells in seminiferous tubules of testes

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3
Q

semen components

A

fructose, flavin, proteins, prostaglandins, citric acid, zinc, enzymes (ACID PHOSPHATASE)

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4
Q

Bulbourethral glands

A

secrete fluid that lubricates the penis & neutralizes urethra

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5
Q

Testes & epididymis

A

sperm produced in testes & mature in epididymis

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6
Q

Prostate

A

FIRST PORTION OF EJACULATE- thin milky fluid
contains most of the sperm!!!!
acidic
contains acid phosphatase & proteolytic enzymes resulting in liquefaction

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7
Q

Seminal vesicles

A 
LAST FRACTION OF EJACULATE
major contributor of volume
alkaline
enzymes necessary for coagulation
supplies fructose, flavin & other nutrients
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8
Q

collection

A

several samples over 3 month period
2-7 days abstinence
deliver to lab within one hour maintained at body temp

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9
Q

appearance

A

normal: homogeneous, gray-white, opalescent
abnormal: clear, red, yellow green etc

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10
Q

normal coag & liquefaction

A

completely liquefied within 60 minutes at room temp

can treat with chymotrypsin if not completely liquefied

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11
Q

normal volume

A

> 1.5 ml

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12
Q

normal viscosity

A

semen falls from pipet in drops or threads are

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13
Q

normal pH

A

> 7.2

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14
Q

normal motility

A

50% or more of sperm should show moderate to strong linear or forward progression
>32% progressively motile (!!!!!!!!!)

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15
Q

Sperm concentration

A

dilute specimen & perform count on hemocytometer

normal: 15-150 mil/ml

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16
Q

calculation for sperm concentration

A

cells x dilution
———————–x 1000
volume

17
Q

total sperm count

A

sperm concentration x total volume

normal > 39x10^6 / ml

18
Q

stains

A

Wright, Giemsa, Papanicolauo stains

19
Q

morphology

A

stain use 100x oil, observe 200 sperm

normal >4% normal morphology

20
Q

head

A

oval
2.5-3.5 um wide & 4.0-5.0 um long
head to length ratio should be 1.5-1.75

21
Q

midpiece

A

6-7.5 um long & thicker than tail but

22
Q

tail

A

slender, uncoiled @ least 45 um long

23
Q

sperm vitality

A

done when motility

24
Q

agglutination

A

even small amount is abnormal
production of anti-sperm antibodies
clumping = bad

Urinalysis & Body Fluids (8 decks)

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