Seminal Fluid Flashcards
Where are sperm produced?
produced in seminiferous tubules + fluid in vas deferens, then passes through epididymis
T/F at home delivery of sperm must be delivered within 2 hr and kept warm
false, must be 1 hr and warm
What is the avg abstinence required for sperm collections
2-3 days of abstinence no longer than 7
What time should the specimen liquify in?
20-60 min roughly
What is the normal color of appearance? Infection? Trauma? Abnormal?
normal: white/grey translucent
infection: turbid (culture)
trauma: bloody abnormal
T/F ejaculation that is yellow means there is urine present, limiting motility and could be caused by prolonged abstinence/medications
True
T/F for clots remaining after 1hr, wait for liquification before analyzing
true
What is the normal volume of sperm? what would decreased volume mean?
avg 3.5mL
2-5mL
Decreased: infertility, incomplete collection
What is normal viscosity for sperm collection?
droplets with thin threads normal
rates from 0-4
What is the normal pH of spermies
7.2 - 8.0
>8.0 infection, high acid = increased prostate fluids
Normal sperm concentration
> 20 million/mL - 250 million/mL
Normal sperm count
> 40 million per ejaculate
Sperm counted on a automated neubauer: how many squares are counted, dilution?
same as rbcs - count 5 secondary squares
dilution 1:20
T/F clumps seen in sperm do not indicate anti-sperm Ab
false, it does indicate anti-sperm Ab
what would be considered good motility for sperm?
forwards progressive movement, examine within 1hr, eval undiluted slide
T/F normal motility is rated as 50% slow forward/more lateral movement
true
how many sperm do you count for morphology?
200 on each side, 400 in total
What is the normal % morphology? strict criteria morphology?
normal: >30%
strict: >14%
What stain do you use to test viability?
eosin-nigrosin stain
What color do dead sperm stain? normal?
dead sperm stain red, normal stain blue/white
Whats the normal amount of living sperm in a sample?
75% living
