Seminal Fluid Flashcards

1
Q

Where are sperm produced?

A

produced in seminiferous tubules + fluid in vas deferens, then passes through epididymis

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2
Q

T/F at home delivery of sperm must be delivered within 2 hr and kept warm

A

false, must be 1 hr and warm

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3
Q

What is the avg abstinence required for sperm collections

A

2-3 days of abstinence no longer than 7

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4
Q

What time should the specimen liquify in?

A

20-60 min roughly

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5
Q

What is the normal color of appearance? Infection? Trauma? Abnormal?

A

normal: white/grey translucent
infection: turbid (culture)
trauma: bloody abnormal

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6
Q

T/F ejaculation that is yellow means there is urine present, limiting motility and could be caused by prolonged abstinence/medications

A

True

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7
Q

T/F for clots remaining after 1hr, wait for liquification before analyzing

A

true

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8
Q

What is the normal volume of sperm? what would decreased volume mean?

A

avg 3.5mL
2-5mL
Decreased: infertility, incomplete collection

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9
Q

What is normal viscosity for sperm collection?

A

droplets with thin threads normal
rates from 0-4

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10
Q

What is the normal pH of spermies

A

7.2 - 8.0
>8.0 infection, high acid = increased prostate fluids

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11
Q

Normal sperm concentration

A

> 20 million/mL - 250 million/mL

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12
Q

Normal sperm count

A

> 40 million per ejaculate

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13
Q

Sperm counted on a automated neubauer: how many squares are counted, dilution?

A

same as rbcs - count 5 secondary squares
dilution 1:20

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14
Q

T/F clumps seen in sperm do not indicate anti-sperm Ab

A

false, it does indicate anti-sperm Ab

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15
Q

what would be considered good motility for sperm?

A

forwards progressive movement, examine within 1hr, eval undiluted slide

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16
Q

T/F normal motility is rated as 50% slow forward/more lateral movement

A

true

17
Q

how many sperm do you count for morphology?

A

200 on each side, 400 in total

18
Q

What is the normal % morphology? strict criteria morphology?

A

normal: >30%
strict: >14%

19
Q

What stain do you use to test viability?

A

eosin-nigrosin stain

20
Q

What color do dead sperm stain? normal?

A

dead sperm stain red, normal stain blue/white

21
Q

Whats the normal amount of living sperm in a sample?

A

75% living