Semester 1 Final Flashcards
Science
A particular way of knowing about the world
Independent variable
What the experiment will deliberately change or manipulate in the investigation
Dependent variable
What changes in response to the independent variable
Control
Group used for comparison with your experiment. This is the “normal group”
Constants
The aspects of an experiment that are held consistent
Objective
Verifiable observation
Ex: water boils at 100 degrees Celsius
Subjective
Based on personal opinions and feelings, NOT FACTS
Quantitative
Uses numbers to measure something
Qualitative
Describes qualities
Phospholipid bilayer
Thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells
Hydrophilic
Likes water
Hydrophobic
Repels from water
Concentration
Amount of solute dissolved in solvent
Concentration gradient
Particles move from high concentration to low concentration (no energy needed)
Passive transport
Requires no extra energy
Active transport
Requires extra energy to be spent
Simple Diffusion
The spreading out of molecules across a membrane until equilibrium is reached
Facilitated diffusion
A transport protein helps to facilitate diffusion of molecules that normally couldn’t pass through the cell membrane
Osmosis
The simple diffusion of water across the cell membrane
Solution
Uniform mixture of two or more substances
Solute
What gets dissolved
Solvent
Does the dissolving
Homeostasis/Equilibrium
Balanced
Isotonic
Identical water to cells cytoplasm ->
Cell stays the same
Hypertonic
Water is lower that the cell’s cytoplasm -> net movement of water out of the cell (cell shrivels)
Hypotonic
Water is higher than the cell’s cytoplasm -> net movement of water into a cell (cell swells)
Selective permeability
Ability to differentiate between what types of molecules can pass through the cell membrane
Gene
Segment of DNA for a specific trait
Ex: brown eyes
Genetic code
Instructions in a gene to make that specific protein
Genome
All genetic material in an organism
Chromosome
Supercoiled threadlike structure of DNA and protein that contains genetic information.
DNA
Deoxyribonucleic acid. A molecule that contains the genetic coding necessary for reproduction of living cells.
Double helix
Shape of DNA
5’
Phosphate on the end
3’
Sugar on the end
Antiparallel
Parallel but moving in different directions (5’-3’)
RNA
Ribonucleic acid. Helix shape
Adenine
Double ringed purine
Guanine
Double ringed purine
Cytosine
A single ringed pyrimidine
Thymine
A single ringed pyrimidine
Purine
Nitrogenous base with connected 6 and 5 sided ring
Pyrimidine
6 sided nitrogenous base
Chargoff’s Rule
The bond between A=T & C=G. The statement that some nitrogenous bases have relatively the same amount as others
Covalent Bond
Strong bonds that connect the bases,sugar, and phosphate groups in DNA
Hydrogen Bond
Weaker than covalent bonds. Connect GC and AT
Helicase
- Enzymes that unwinds DNA by breaking the hydrogen bonds
- Separates DNA into single strands
DNA Binding Proteins
- Binds to DNA after it has been split to keep the two strands apart
- Prevents bonds from reforming
Replication Fork
- Space where replication occurs
- Moves with helicase
RNA Primase
- Identify and signal the starting point(s) of where DNA replication should begin
- Provides a landing site
- Placed only on single stranded DNA as close to the 3’ end as possible
DNA Polymerase
- MVP
- Enzyme that scans the original DNA strand and adds complementary nucleotides
- Must add from 5’ -> 3’
Free Nucleotides
- Phosphate, sugar, nitrogenous base
- Floating around in nucleus
- Building blocks of new DNA
Leading/Lagging Strand
- DNA is replaced on both strands of DNA at the same time
- Polymerase moves along the original template (3’-5’, Adds new DNA 5’-3’)
- Leading = DNA polymerase works in same direction as helicase (continuous)
- Lagging = DNA polymerase works in opposite direction as helicase (discontinuous)
Okazaki Fragments
- Short chunks of newly formed DNA on the lagging strand
- Results from the discontinuous addition of new nucleotides
DNA Ligase
- Enzyme that glues Okazaki fragments together by adding covalent bonds between sugars and phosphates
- Converts the discontinuous strand into a continuous strand