Semen Evaluation And AI Flashcards
What body condition scoring scale is used at NCSU beef unit?
1-9
1=emaciated
9=very obese
We want Cattle to be 5-6
At what temperature are sperm stored in a liquid nitrogen refrigerator?
-320 Fahrenheit
Why aren’t sperm cells destroyed in this extremely cold environment?(liquid N)
Sperm cells are placed in an extender containing a cryopreservant, and sperm are rapidly frozen which prevents formation of ice crystals
What is the critical danger zone?
the neck region of the liquid nitrogen refrigerator, where the temperature rapidly increases
At what temperature and for how long do we thaw a semen straw?
Thaw semen at 95F (35C) for 30-45 seconds
What are the 6 main areas that we observe in cattle to determine a body condition score?
tail head, back, hooks, pins, ribs, and brisket
What is capacitation and why is it important?
final maturation step of spermatozoa; a biochemical event that alters the sperm head,induces hypermotility and enables the sperm cell to attach and penetrate the egg
What is the volume and sperm concentration of an AI straw?
volume is 0.5 cc (mL) and 20 to 40 million sperm per straw
How does time and temperature impact semen quality post-thaw?
Changes in temperature either too cold or hot will kill the sperm, additionally, We observed that sperm motility decreased over time post-thaw
Which species has the easiest semen to preserve?
Cattle, Dairy Cattle are the leading species where AI is used; therefore, a lot of research has been focusing on preserving cattle semen
What information can we find on a semen straw?
AI Center code (1-312)
Bull’s Breed (AN)
Bull’s ID within AI center (100)
Why must we use fresh semen when using AI in boars?
freezing boar semen causes a significant loss of motility
A nitrogen tank can hold up to _______ straws
12,000
Before straws, semen was packaged in…
ampules
Unit that holds semen straws; attaches to the cane
Goblet
