Selection Practical 1 Flashcards
Which type of selection markers were used
Amp resistance in plasmids
Lac Z ability to digest x gal
What is the screening called
Blue white screening
Which types of plasmid transformants grow on the amp plate
Both pic19h and pblt101 (not disrupted)
What does lac z encode for in the pic19h
A peptide and b galactosidase
What is the function of b galactosidase
Breaks lactose glycosidic bonds for carbon source (gal and glucose produced)
When glucose is low camp binds to cap proteins which allow lac z promoter recognition
What is kept constant in the blue white screening to stop other factors
Iptg (removes the lac repressor from operator)
Why do pblt101 transformers stay white
No functioning lac z as the insert is added into the mcs of lac z
No b gal
No breaking of x gal glycosidic bonds
No oxidisation
Stays white
