Restriction Check Prac 1

Question 1

Why is restriction digestion done on both pblt101 and pic19h plasmids post transformation

Answer 1

To see and confirm which ecoli were successfully transformed using the ecor1 and then run on agarose gel

Question 2

Which 4 samples are used for the restriction analysis

Answer 2

1- uncut pic19h (without ecor1) : from the miniprep

2- cut pic19h (with ecor1)

3- uncut pblt101

4- cut pblt101 (would show only insert)

Question 3

How many ecor1 sites does pic19h have and what does this mean

Answer 3

1

This means it is cut into linear fragments and produces bands