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HistoPath Lab 2ndYR 2ndSem Finals > Sectioning > Flashcards

Sectioning Flashcards

1
Q

Microtome model that we use in the lab

A

Leica RM2125 RTS Microtome

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2
Q

Measurement of sections produce by the Leica RM2125 RTS Microtome

A

0 - 60um

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3
Q

2 dot meaning

A

Initial Sectioning

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4
Q

Initial sectioning measurement

A

25um

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5
Q

1 dot meaning

A

Final Sectioning

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6
Q

Final sectioning measurement

A

10um

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7
Q

Floatation bath temp

A

5-10C below melting point of paraffin wax

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8
Q

Most commonly used adhesive

A

Meyer’s Egg Albumin

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9
Q

Ratio concentration for Meyer’s Egg Albumin

A

1:1

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10
Q

Meyer’s Egg Albumin composition

A

1mL Egg white & 1mL Glycerin

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11
Q

Added to Meyer’s Egg Albumin to prevent molds

A

Phenol

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12
Q

Adding Phenol to Meyer’s Egg Albumin will allow the slide to last for how long?

A

3 - 4 months

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13
Q

Other names for Sectioning

A

Microtomy
Cutting

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14
Q

The process where tissues are cut into uniformly thin slices to facilitate microscopic studies

A

Sectioning

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15
Q

Most paraffin embedded tissues are sectioned to what measurement

A

4 - 8um

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16
Q

Histotechnologists are the _______ of the laboratory

A

artists

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17
Q

Common artifacts introduced in sectioning

A

Tearing
Ripping
Holes
Folding

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18
Q

4 types of microtome knives

A

Standard Thick Metal
Thin Disposable Blades
Glass Knives
Diamond Knives

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19
Q

This knife allows for sharpening to your liking and is considered to be expensive

A

Standard thick metal

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20
Q

Does the same job as the standard thick metal knife, and probably better, for a much lesser cost

A

Thin disposable blades

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21
Q

Glass knives are for tissue sections embedded in what types of plastic materials

A

Methacrylate
Araldite
Epon

22
Q

A glass knife can section down to what measurement

A

About 1um

23
Q

Knife used for electron microscopy

A

Diamond knives

24
Q

Diamond knife can section to what measurement

A

About 0.25um

25
Q

Angle that prevents the contact between the knife facet and the face of the block

A

Clearance angle

26
Q

Recommended clearance angle

A

0 - 15 degrees

27
Q

Bevel angle is aka?

A

Facet angle

28
Q

The angle between the two facets that form the cutting edge

A

Bevel Angle

29
Q

Facet angle

A

approx. 35 degrees

30
Q

Consequences of incorrect knife angle will vary on the differences in what factors?

A

Specimen types
Fixation
Processing Qualities
Knife Flexibility

31
Q

Causes if the ribbon is crooked rather than straight

A
  • edges of the block face are not parallel to each other
  • uneven knife edge
32
Q

Causes if the ribbon will not form and each section comes off separately

A
  • Dull blade edge
  • Knife has wrong tilt
  • Edges of paraffin block face are crumbled or rounded
33
Q

Causes if sections are compressed or folded

A
  • dull knife
  • knife angle is set too close to the vertical
  • knife is clogged with paraffin
  • sections are too thin
  • paraffin is too soft due to warm room
  • paraffin is contaminated with xylene
34
Q

Causes if the specimen crumbles or falls out of the paraffin section

A
  • tissue is inadequately dehydrated or cleared
  • tissue is too hard and compact
  • tissue was in the paraffin oven too long and at too high temp
  • tissue is too hard for paraffin matrix
35
Q

Causes if the ribbon continuously splits or scratches vertically

A
  • crystals or dirt particles are caught on the knife edge or on paraffin face
  • nicked knife edge
36
Q

Causes if the ribbon wraps itself around your finger, the knife blade, or anything else

A
  • static electricity
37
Q

In prepping the tissue block for sectioning, trimming the lateral surface should result in resembling what shape

A

Trapezoid

38
Q

After resulting in a trapezoid resembling shape, the tissue block must still be surrounded by a frame of how much wax

A

2-3mm

39
Q

Wipe the microtome blade using what solution?

A

Chloroform or Xylene

40
Q

In the blade preparation, the angle of the center line of the blade with the face of the block should be about how many degrees

A

20 degrees

41
Q

During sectioning, students should start at how many microns

A

10um

42
Q

In sectioning, how much paraffin should be allowed to move up and down until the ribbon begins to bow up

A

first inch or two

43
Q

How long must the ribbon be before detaching it form the edge of blade

A

8 inches

44
Q

How must we detach the ribbon from the edge of the blade

A

In an upward stroke

45
Q

How should we brush the blade

A

away from the cutting of the blade

46
Q

What should the orientation of the ribbon be when laying it in the ribbon box

A

Dull surface up

47
Q

When mounting sections, slide should be rinsed in what

A

95% ethanol

48
Q

What should be used to write information on the slide

A

Diamond-tipped pencil
Lead pencil

49
Q

Paraffin expands to how many percent

A

20%

50
Q

What is the measurement of the cover slip used after staining

A

22mm x 22mm

51
Q

At what temperature should the slide warmer be

A

40 - 45C

HistoPath Lab 2ndYR 2ndSem Finals (2 decks)

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