H&E Staining

Question 1

Stains nucleus

Answer 1

Hematoxylin

Question 2

Stains cytoplasm

Answer 2

Eosin

Question 3

Unstained tissue section under the microscope

Answer 3

Dull grey color

Question 4

Dye used for routine microanatomical examination

Answer 4

Hematoxylin & Eosin

Question 5

Staining method of choice

Answer 5

H&E

Question 6

Introduced H&E in 1865

Answer 6

Bohmer

Question 7

Introduced H&E in 1875

Answer 7

Fischer

Question 8

Described H&E as a tissue staining method for staining different materials with different colors

Answer 8

Wissowzky (1876)

Question 9

Most important type of bonding that occurs in histologic staining techniques

Answer 9

Ionic Bonding

Question 10

Hematoxylin needs to be used in combination with what

Answer 10

Mordant

Question 11

Compound that helps hematoxylin link to the tissue

Answer 11

Mordant

Question 12

A typical mordant

Answer 12

Metal Cation (Aluminum)

Question 13

Cationic dye

Answer 13

Hematoxylin

Question 14

Anionic dye

Answer 14

Eosin

Question 15

Color that Hematoxylin stains

Answer 15

Blue

Question 16

Color that Eosin stains

Answer 16

Pink

Question 17

Used for the removal of wax in deparaffinizatrion

Answer 17

Xylene

Question 18

Appearance of sections after hydration

Answer 18

Opaque

Question 19

Mercury is treated with iodine solution which changes it to what

Answer 19

Iodine Compounds

Question 20

Mercury is changed to an iodine compound which is then converted to?

Answer 20

Tetrathionate

Question 21

Iodine compound is converted to Tetrathionate by what

Answer 21

Thiosulphate

Question 22

Starting concentration of alcohol in Hydration

Answer 22

Absolute alcohol (100%)

Question 23

Starting concentration of alcohol in Dehydration

Answer 23

70%

Question 24

How long slides should cool from the slide warmer

Answer 24

at least 5 mins

Question 25

Number of changes and for how long is the first Xylene

Answer 25

2 changes
5 minutes each

Question 26

Number of dips for Alcohol formol

Answer 26

10 dips

Question 27

Number of changes and dips for 95% Ethanol

Answer 27

3 changes
10 dips each

Question 28

Used to wash slides

Answer 28

Soapy Water

Question 29

Staining time for Hematoxylin

Answer 29

5-10mins

Question 30

Number of dips for Acid Alcohol

Answer 30

2 quick dips

Question 31

2 Differentiators

Answer 31

Acetic Acid
Acid Alcohol

Question 32

Time for Acetic Acid differentiator

Answer 32

45 seconds / 2 dips

Question 33

Time for Acid Alcohol differentiator

Answer 33

1min

Question 34

Immersion time of Ammonia Water

Answer 34

avg. of 5 mins (or until tissue turns blue)

Question 35

Scott’s Tap water composition

Answer 35

20g Mg Sulfate
3.5g Na Bicarbonate
1L distilled water

Question 36

Acid Formol composition

Answer 36

Formalin
Acetic Acid
Distilled Water
100% Ethanol

Question 37

Staining time for Eosin Y

Answer 37

1.5 - 3mins

Question 38

Number of changes and dips for second Xylene

Answer 38

3 changes
10 dips each

Question 39

Basic Steps in Staining and Mounting Paraffin sections

Answer 39

1. Deparaffinization
2. Hydration
3. Removal of Mercury Pigments
4. Staining
5. Dehydration & Clearing

Question 40

Presence of patches indicates presence of what

Answer 40

Wax

Question 41

Presence of any clear areas are indicative of the presence of?

Answer 41

Xylene

Question 42

Example of Mercury containing fixative

Answer 42

Zenker’s Fluid