section B

Question 1

discuss the importance of good lab practice in a microbiology lab

Answer 1

1.safety: protects people in the lab from chemicals and harmful microorganisms
2.accuracy: ensures reliable and reproduceable experimental results
3.contamination: prevents cross contamination of samples and environment
4.efficiency:reduces errors ensuring accurate results

Question 2

four routes of infection in a microbiology laboratory

Answer 2

1.inhalation:breathing in aerosols from cultures or spills
2.ingestion:accidental ingestion from contaminated hands or surfaces
3.injection:needle or sharp instrument injury causing pathogen entry
4.skin contact: direct skin contact with contaminated surfaces or cultures

Question 3

discuss the process of binary fission

Answer 3

1.DNA replication: the bacterial DNA is copied
2.cell elongation: the cell grows and and the two copied DNA move to opposite ends.
3.septum formation: a division septum forms in the middle of the cell.
4.cell:the septum completes and splits two identical daughter cells.

Question 4

what are the three factors that affect microbial growth

Answer 4

pH
Temperature
Oxygen