Section 3

Question 1

DNA polymerase

Answer 1

enzyme that copies a DNA template, catalyzes the joining of dNTP’s (add a dNTP to the 3’ hydroxyl group) . DNA poly I, DNA poly III, DNA poly (α) alpha , DNA poly (δ) delta,DNA poly (ε) epsilon, DNA poly (γ) gamma. Needs primer to synthesis DNA

Question 2

DNA polymerase I

Answer 2

1st DNA polymerase to be identified in E.coli, mostly helps with repairing damaged DNA

Question 3

DNA polymerase I

Answer 3

1st DNA polymerase to be identified in E.coli, mostly helps with repairing damaged DNA. REmoves RNA primers from okazaki fragments. Can act as exonuclease that hydrolize DNA or RNA.— 5’ - 3’ exonulease removes ribonucleotides from 5’ ends of Okasaki fra,, replaced by deoxynuc

Question 4

DNA poly α

Answer 4

functions in replication of nuclear DNA in Eukaryotes

Question 5

DNA poly α

Answer 5

FOund in a complex w/ primase and works w/ it to make short RNA-DNA fragments for Oris and during lagging strand. functions in replication of nuclear DNA in Eukaryotes

Question 6

DNA poly (δ) delta

Answer 6

LAGGING:: Fills in gaps between okazaki fragments, MAjor function in replication of nuclear DNA in Eukaryotes.

Question 7

DNA poly (ε) epsilon

Answer 7

LEADING:: MAjor function in replication of nuclear DNA in Eukaryotes

Question 8

Replication forks

Answer 8

Regions of active DNA synthesis for daughter strands

Question 9

DNA ligase

Answer 9

Joins the Okazaki fragments at their niks–forms new intact DNA strand.

Question 10

DNA ligase

Answer 10

DNA poly δ fills at their gaps while ligase joins resulting fragments –forms new intact DNA strand.

Question 11

RNase H

Answer 11

Removes RNA primers in eukaryotes. Enzyme that degrades RNA strand of RNA-DNA hybrids and 5’-3’ exonucleases

Question 12

Sliding-clamp Proteins/(PCNA) proliferating cell nuclear antigen

Answer 12

Act at the replication fork. Binds to DNA poly III in Prok and DNA poly δ and ε in Euk Load the poly onto primer and maintain bound to template.. Forms ring around template DNA

Question 13

CLamp loading proteins/ Replication factor C (RFC)

Answer 13

Loads PCNA between primer and template in rep fork uses energy from ATP hydrolysis to open clamps.

Question 14

CLamp loading proteins/(RFC) Replication factor C

Answer 14

Loads PCNA between primer and template in rep fork uses energy from ATP hydrolysis to open clamps.

Question 15

Helicase

Answer 15

Enzyme, unwinds parent DNA, coupled to hydrolysis of ATP

Question 16

Single-Stranded DNA-binding proteins/(RPA) Replication Protein A

Answer 16

Stabilize the unwound template DNA. Keep

Question 17

Topoisomerase

Answer 17

Topoisomerase I, TopoisomeraseII. Ezymes that catalyze the reversible breakage and rejoining of DNA strands.

Question 18

Topoisomerase I

Answer 18

Breaks 1 strand,

Question 19

Topoisomerase II

Answer 19

Breaks 2 strands, unravels newly replicated DNA that interwine w/ eachother