Secondary hemostasis - lab Flashcards
secondary hemostasis happens at the __
platelet membrane
secondary hemostasis happens at the platelet membrane, the fluid phase inhibited by __
coagulation control proteins
enumerate the classification of blood factors
- substrate
- zymogen
- serine protease
- cofactor
- calcium
substance where an enzyme acts
substrate
an enzyme precursor and the inactive forms of a coagulation factor
zymogen
serine protease is the proteolytic enzymes of __ and with the __ residue in active sites
trypsin family, reactive seryl amino acid
t or f: cofactors needs to be present in addition to the enzyme so a particular reaction will be catalysed.
true
binds serine protease
cofactor
needed for activation of coagulation factors
calcium
calcium activates the coagulation factors except the __
contact phase
flips to the outer surface on activation and is the phospholipid surface on which clotting enzymes assemble and is the phospholipid surface on which clotting enzymes assemble
phosphatidylethanolamine
enumerate the factor/s present in common pathway
X, V, prothrombin, fibrinogen
enumerate the factor/s present in intrinsic pathway
VIII, IX, XI, XII, prekallikrein, HMWK
enumerate the factor/s present in extrinsic pathway
VII
enumerate the factors present in absorb plasma
I, V, VIII, XI, XII, XIII, prekallikrein, HMWK
enumerate the vitamin k dependent factors
II, VII, IX, X, XI
enumerate the name of these coagulation factors in which, the active form is a cofactor
V - proaccelerin
VIII - antihemophilic factor
High molecular weight kininogen
active form of fibrinogen
fibrin
active form of factor II
thrombin
active form of factor VII
serine protease
enumerate the name of these coagulation factors in which, the active form is a serine protease
VII - proconvertin
IX - plasma thromboplastin component
X - stuart-power factor
XI - plasma thromboplastin antecedent
XII - hageman
XIII - fibrin stabilizing factor
prekallikrein
enumerate the 3 stages of secondary hemostasis
1: Generation of thromboplastin
2: conversion of prothrombin to thrombin
3: conversion of fibrinogen to fibrin clot
stage 1 - generation of thromboplastin is also known as the
enzymatic phase
stage 2 - conversion of prothrombin to thrombin is also know as the
polymerization
stage 3 - conversion of fibrinogen to fibrin clot is also known as the
coagulation/clotting
a factor produced endothelial cells and megakaryocytes
von willebrand
vWf is stored in a-granules of platelets and in ___ of endothelial cells
Weilbel-Palade bodie
formation of complexes: TENASE
factor IXa, XIII, Calcium and PF3
formation of complexes:
factor Xa, Va, calcium , PF3
prothrombinase
formation of complexes
- TF, factor VIIa, phospholipids, calcium
- factor IXa, factor XIII, Calcium and PF3
- factor Xa, factor Va, calcium , PF3
pathway that stars at factor XII and culminates in fibrin polymerization
intrinsic pathway
extrinsic pathway is also known as the
tissue factor pathway
it is where both pathway meets and ends in formation of fibrin clot
common pathway
fibrinogen group is also known as the
thrombin sensitive group
fibrinogen: calcium and vit k
calcium dependent; vit K independent
fibrinogen involves what factor?
I, V, VIII, XIII
fibrinogen group: plasma and serum
present in plasma, absent in serum
fibrinogen is increased in __
- pregnancy
- inflammatory response
- women on oral contraceptives
- stress
what factor/s decreased in in-vitro storage
V and VIII
prothrombin group involves what factor/s?
II, V, VII, IX, X, protein C and S
prothrombin group: calcium and vit K
calcium and vit K dependent
necessary for the synthesis of prothrombin in the liver
vitamin K
prothrombin group: plasma and serum
present in serum and plasma
prothrombin is present in serum and plasma except?
except factor II
competes with vit K
coumarin drugs
this require a calcium or a cofactor to bind with phospholipid surfaces
prothrombin
factor/s activated in cold temp
VII, XI
examples of vit K sources
leafy vegetables, bacteriodes fragilis, e. coli
contact group: calcium and vit K
calcium and vit K independent
contact group involves what factor/s?
XII, XI, Prekallikrein, HMWK
not adsorbed, not consumed during coagulation
contact group
contact group is relatively stable and requires contact of __ for activation
negative-charged surface
these factor/s function in the activation of plasminogen and fibrinolytic system
prekallikrein and HMWK
clot retraction: after the fibrin clot has been stabilized by what factor?
factor XIIIa
