Second Week Flashcards
Bacterial genetics, pathogenecity and biochemichal therapy+virus
Library screening identify colonies which have specific:
Select one:
a. RNA
b. Gene
c. DNA
d. Bacteria
Bacteria
Why it is important to use restriction enzymes with sticky ends for cloning?
Select one:
a. For easy identification of the recombinant plasmid
b. For easy insertion of DNA fragments into the plasmid vector
c. For easy identification of plasmids with antibiotic marker
d. For easy transformation
b.For easy insertion of DNA fragments into the plasmid vector
You are inserting gene fragment into a plasmid containing ampicillin resistance gene. The plasmid was transformed into bacteria that are kanamycin resistant. Which of the following is the best medium to select the bacteria containing the recombinant plasmid?
Select one:
a. A medium containing no antibiotics
b. A medium containing both ampicillin and kanamycin
c. A medium containing only kanamycin
d. A medium containing streptomycin
b.A medium containing both ampicillin and kanamycin
Which of the following choices lists the steps of pathogenesis in the correct order?
Select one:
a. adhesion, exposure, infection, invasion
b. disease, infection, exposure, invasion
c. exposure, adhesion, invasion, infection
d. invasion, infection, adhesion, exposure
exposure, adhesion, invasion, infection
Which of the following would be a virulence factor of a pathogen?
Select one:
a. a surface protein the host immune system recognizes
b. a secondary host the pathogen can infect
c. the ability to grow
d. surface protein allowing the pathogen to bind to host cells
surface protein allowing the pathogen to bind to host cells
Which part of a microbial cell causes an endotoxin?
Select one:
a. Flagella
b. Pili
c. Cell wall
d. Cell membrane
Cell wall
Presence or persistence of microorganism in/or on the body is termed:
Select one:
a. colonization
b. virulence
c. commensals
d. toxigenicity
a.colonization
E. coliattaches to host tissue through:
Select one:
a. flagella.
b. cell membrane
c. cell wall
d. fimbriae
fimbriae
What is defined as the number of microbes required to produce a disease
Select one:
a. Pathogenesis
b. Infection
c. Virulence
d. Infectious Dose
e. Pathogen
Infectious Dose
You have recently identified a new toxin. It is produced by a gram-negative bacterium. It is composed mostly of protein, has high toxicity, and is not heat stable. You also discover that it targets liver cells. Based on these characteristics, how would you classify this toxin?
Select one:
a. leukocidin
b. superantigen
c. exotoxin
d. endotoxin
exotoxin
Which of the following is an enterotoxin?
Select one:
a. Tetanospasmin
b. Botulinum toxin
c. Diphtheriae toxin
d. Toxin ofE. coli
Toxin ofE. coli
Collagenase causes damage to which of the following?
Select one:
a. Lecithin
b. White blood cells
c. Connective Tissue
d. Red blood cells
Connective Tissue
To establish an infection, a bacterial cell:
Select one:
a. must adhere to, colonize and/or invade the host
b. must stay at the outside of the body surface
c. always secretes extotoxin
d. must secrete endotoxin
must adhere to, colonize and/or invade the host
Toxoid is …..
Select one:
a. None are correct
b. Toxin lost its toxicity but still antigenic
c. Toxin lost toxicity and antigenicity
d. Toxin lost its antigenicity but not toxicity
Toxin lost its toxicity but still antigenic
Which of the following antibiotics specifically inhibits DNA synthesis?
Select one:
a. Tetracycline
b. Gentamycin
c. Polymyxin B
d. Quinolones
Quinolones
Sulfonamides inhibit the production of folic acid and, therefore, inhibit the synthesis of __________.
Select one:
a. Purines and pyrimidines
b. Ribose
c. Peptidoglycan
d. Aromatic amino acids
Purines and pyrimidines
Which of the following is not associated with every virus?
Select one:
a. capsomers
b. envelope
c. nucleic acid
d. capsid
e. genome
envelope
Viruses that consist of only a nucleocapsid are considered
Select one:
a. simple viruses
b. incomplete viruses
c. naked viruses
d. viroids
e. complex viruses
naked viruses
Which of the following viruses comprises diploid genome?
Select one:
a. Rabies virus
b. Retrovirus
c. Rotavirus
d. Reovirus
Retrovirus
The proteins on the external surface of the viruses serve several important functions. Regarding these proteins, which one of the following statements is most accurate?
Select one:
a. They are antigens against which neutralizing antibodies are formed
b. They are the polymerases that synthesize viral messenger RNA
c. They are the proteins that initiate viral transcription
d. Change in the conformation of these proteins can result in prion mediated diseases
e. They are the proteases that degrade cellular proteins leading to cell death
They are antigens against which neutralizing antibodies are formed
Many viruses are highly specific regarding the type of cells they infect. Of the following, which one is the most important determinant of this specificity?
Select one:
a. The polymerase in the virion
b. The viral mRNA
c. The protease protein
d. The surface glycoprotein
e. The matrix
The surface glycoprotein
Retroviruses achieved their long term infection status only by which of the following?
Select one:
a. The viral genome stays in the neuron in forms of episome
b. Maintenance of the whole virus particle in a neuron
c. The viral genome stays in the epithelial cells in forms of episome
d. Integration of the viral nucleic acid into the host DNA
Integration of the viral nucleic acid into the host DNA
Which of the following viruses is the largest of all animal viruses?
Select one:
a. Poxviruses
b. Picornaviruses
c. Retroviruses
d. Herpesviruses
Poxviruses
