second midterm Flashcards

1
Q

Go back and add flashcards from the last chapter of mineral nutrients and the first part of solute transport

A

deal. will do

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2
Q

What form is Fe usually found / used? in

A

Fe3+, iron is largely oxidized and insoluble

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3
Q

What is interveinal chlorosis?

A

the characteristic symptom of iron deficiency

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4
Q

What is chelation?

A

the formation of bonds between two or more seperate binding sites within a ligand and a single central atom
- a central metal atom attactched to a ligand in a cyclic structure

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5
Q

What is the tonoplast?

A

the membrane of the central vacuole

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6
Q

Define chemical potential

A

The energy (ability) of a substance to perform (move) freely work

  • the sum of the concentration, electrical and hydristatic potentials under standard conditions
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7
Q

specific definition of diffusion?

A

movement of molecules from a region of high concentration or high chemical potential to an area of low concentration or low chemical potential

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8
Q

What is facilitated diffusion?

A
  • involves the movement of specific molecule / ions
  • needs specific channels or carrier proteins
  • does not use ATP for transport - it is passive transport
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9
Q

Which requires a greater gibbs free energy, simple or facilitated diffusion?

A

simple diffusion is less efficient because the membrane acts as a barrier and kinda slows it down
- facilitated diffusion is more efficent - has a lower gibbs free energy

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10
Q

When does simple diffusion overtake facilitated diffusion?

A

facilitated diffusion has a much higher rate of transport at low concentrations - but will plateau at some point when all membrane transporters / membrane proteins are fully saturated at the high concentration. As long as the area or concentration gradient does not change simple diffusion will eventually overtake facilitated diffusion in transport rate

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11
Q

How does diffusion continue once the solute has reached an equilibirum on either side of the membrane?

A

for example, metabolic consumption of glucose will lower the concentration of glucose on one side of the membrane - then the concentration gradient will be restablished - like that one chem thing of removing a reagent to push the equilibirum forward
- facilitated diffusion will continue

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12
Q

Two examples of passive transporters in plants

A

Plastid glucose transporter in the chloroplast inner envelope
triose phosphate translocator in the stoma - mediates a passive counter exchange of Pi and triose phosphate

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13
Q

Explain facilitated diffusion of charged species, ex) KCl

A

If you have K+ and Cl- ions on one side of the membrane, and a K+ transporter, then K+ will move to the other side of the membrane. Diffusion gradient will move K+ across, chemical potential gradient will go across, electrochemical potential gradient will go across, electrical gradient will go back the other way -this system will be in equilibrium even tho the concentrations arent equal

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14
Q

explain the difference between an ATPase, symporter, and anitporter

A

ATPase - is a proton pumping transporter that uses ATP for power

Symporter - couples with ATPase to transport two different ions in the same direction

Antiporter - couples with ATPase to transport two different ions in different directions

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15
Q

Which three mineral elements are transported by symporters?

A

NO3-, Pi (PHT1), and K (HAK)

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16
Q

Which directions do proton pumps usually move protons?

A

usually pump protons outside of the cell

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17
Q

Which side of the plasma membrane is more acidic - based on the gradient generated by proton pumps

A

outside of the cell is more acidic - because

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18
Q

in the current voltage relationship curve, which values are represented by the axis?

A

y axis = flux - positive = efflux, negative = influx
x axis = current in mV

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19
Q

What is the relationship between stomatal aperture and the presence of photosynthetically active radiation?

A

stomatal aperture increases - meaning the stomata open wider - when photosynthetically active light is present
the two are highly correlated and coincides with time of day.

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20
Q

How do guard cells respond to blue light?

A

blue light causes dramatically increased stomatal opening
- stimulated HATPase

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21
Q

you need to come back and look at these slides plz n thanks

A

yes forsolute transport 3`

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22
Q

Why is xylem(?) and phloem considered a non circulatory vascular system?

A
  • xylem transports from root to shoot - in one direction
  • phloem translocates from source to sink - meaning it will move sugars produced by photosynthesis from a source leaf either up into the vegetative meristem or down to the root meristem
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23
Q

What is the pathway for guard cell opening?

A

Blue light triggers HATPase - pumps H out of guard cells - causes K and Cl to accumulate - lowers solute potential - lowers water potential - water uptake into cells - guard cell becomes turgid and the stomata opens

  • blue light also triggers starch degradation and malate synthesis - increases level of sucrose and malate - accumulates sucrose and malate which also lowers solute potential
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24
Q

What is the pathway for guard cell closing?

A

ABA accumulation triggers sucrose to starch conversion - also stops HATPase - opens outward K and Cl channels - sucrose and malate levels decrease - solute potential increases (less solute), water potential increases - water leaves via osmosis - the guard cells become flaccid and close

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25
Q

What is source tissue and what is sink tissue?

A

source tissue - exporting plant tissues or organs that produces photosynthate - sugars - a mature photosynthetically active leaf

sink tissue - non photosynthetic developing organ or an organ that does not produce a significant amount of photosynthate

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26
Q

How are sources and sinks related to tissue maturity?

A

phloem will move sugars from source to sink, or mature tissue to immature tissue - will move from an older leaf to a younger leaf - some leaves are in the middle and represent a source - sink transition leaf

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27
Q

What are the two strong sinks in a plant>

A

vegetative meristem and root meristem - both are young immature tissue and are not differentiated

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28
Q

within one leaf - which area is more mature and therefore a source and which area is less mature and therefore a sink?

A

the leaf tip is more mature / differentiated and therefore is a source - the leaf base is immature and therefore a sink

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29
Q

what is the functional unit of phloem?

A

sieve tubes - functional units for long distance translocation of plant materials - consists of stacked sieve elementsW

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30
Q

What is the sieve plate?

A

perforated wall between sieve elements and maybe also companion cells?

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31
Q

What are four key structures contained in a sieve element?

A
  • structural phloem specific P-proteins
  • endoplasmic reticulum
  • mitochondria
  • sieve element plastids
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32
Q

Which phloem structure contains a nucleus, vacuole and chloroplasts?

A

companion cells

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33
Q

What do P-proteins do in sieve elements?

A

seals off damaged sieve elements by plugging up sieve plate pores
- is a quick plant response and represents a short term solution

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34
Q

What does callose do in the sieve element?

A

seals off damaged sieve elements by plugging up sieve pores
- is a slower plant response but represents a long term solution

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35
Q

Two types of sucrose loading models in minor veins?

A

Symplasmic loading model - sugar moves through plasmodesmata from the mesophyll cells to the phloem

Apoplasmic sucrose loading model - sucrose will move on the outside of plant cells (bundle shealth cell or phloem parenchyma cell) into companion cells - happens via passive transport out of the cell via a permease - and then moves into companion cells by active transport via a sucrose symporter

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36
Q

Can all sugars move through phloem?

A

no, some “reducing” sugars are too reactive to be transported through the phloem

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37
Q

Which sugars will be able to move through the phloem?

A

non reducing - or less reactive sugars can be transported - sucrose

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38
Q

Which sugars cannot move through the phloem and why?

A

sugars with reactive groups like aldehydes and ketones are too reactive - glucose, mannose, fructose

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39
Q

How do plants get around moving reactive sugars through the phloem?

A

will bind other sugars to sucrose or as sugar alcohols
transport as sucrose, raffinose, stachyose, and verbascose

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40
Q

What is being loaded and translocated in phloem?

A

-water
- photosynthate (sugars in specific forms)
- specific amino acids
- ions (K+ and Mg2+
- metabolites
- hormones (auxin, gibberellic acid)
- proteins (signaling and sieve element maintenance)
- RNA (information macromolecules)

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41
Q

Pressure flow mechanism for phloem translocation

A
  1. sucrose is transported into companion cells
  2. sucrose is loaded into sieve tubes from companion cells
  3. sucrose concentration increases in the sieve tubes
  4. water moves into sieve tube and causes pressure to build
  5. this pressure pushes the sucrose laden fluid towards the sink
  6. at the sink sucrose and water are unloaded and distributed among surrounding cells.
  • basically solute concentration is greatest at the source - meaning water will move there and build up pressure as water potential is lowered - pressure will push sugars towards sinks - even though water potential is lower at the source, there is a significant amount of solute in the phloem so pressure is the main acting component. - sink areas have a low turgor pressure bc they are losing water to the xylem as xylem has a higher solute potential
42
Q

Direction and rate of phloem translocation is likely controlled by ____ _____ _______

A

controlled by local sink strength - is not an even split between shoot and root meristem sinks - usually more is transported to the shoot

43
Q

3 reactions of photosynthesis

A

1- photochemical reaction
2- electron trasnfer reaction coupled with formation of ATP and NADPH
3 - biochemical reaction - incorporation of CO2 into carbohydrates (3C sugar)

44
Q

Is the product of photosynthesis glucose?

A

yes, but a better answer is a 3C carbohydrate

45
Q

Is photosynthesis restricted to plants only?

A

No - theres photosynthetic bacteria and cyanobacteria
- single celled organisms like chlamydomonas also have a single large chloroplast

46
Q

What is the light reaction?

A

The first step of photosynthesis - capture of light energy as ATP and reducing power, NADPH
- starts with the low energy oxidized carbon in CO2
- happens in the thylakoid membranes

47
Q

What is the carbon fixing reaction in photosynthesis?

A

The second step of photosynthesis - transfer of energy and reducing power from ATP and NADPH respectively to CO2
- generates a high energy reduced carbon - sugars
- happens in the chloroplast stroma

48
Q

Is photosynthesis an endergonic or exergonic reaction?

A

endergonic - energy in reaction - product is higher energy than the reactants (sugar compared to CO2

49
Q

Is respiration an endergonic or exergonic reaction?

A

exergonic - energy out reaction - substrate has a higher energy than the product (sugar compared to CO2)

50
Q

Is most photosynthesis oxygenic or anoxygenic?

A

Oxygenic -
- removal of electrons from H2O – release O2
- reduction of CO2 to carbohydrate (3C sugar)
- occurs in plants, algae, and cyanobacteria

51
Q

What is anoxygenic photosynthesis?

A

Anoxygenic
- photosynthesis without extracting electrons from water
- Uses H2A instead of H2O
- purple sulfur bacteria use H2S / sulfur

52
Q

Starting with a proplastid, how do you get chloroplasts and chromoplasts?

A

Proplastids - undifferentiated, colorless, found in seeds, meristems and reproductive tissue
Etioplast - photosynthetic tissue but no chloroplhyll - occurs when plastid is grown in the dark
Chloroplast
Chromoplast - red and yellow pigment (carotenoids) found in flowers and fruit

53
Q

Starting with proplastid, what are the three storage plastids you can differentiate into?

A

Amyloplast - starch storage
Elaioplast - storage of oils
Proteinoplast - storage of proteins

54
Q

What does light do to convert etioplast to chloroplast?

A

Light will separate the prolamellar body of the dark etioplast into primary lamellar layers or single thylakoids - which will fuse to form thylakoids and grana layers

55
Q

Grana stacks in thylakoids are a specialty of what kind of plants?

A

land plants

56
Q

WIll plants move their chloroplasts around?

A

Yes, high light means they need to crowd chloroplasts on the top and bottom of the leaf to maximize sunlight uptake
- in conditions where light is too strong, plants will move chloroplasts around side of leaf so that they do not get damaged or overheat from overexposure
- at night chloroplasts move to the bottom of the leaf but the reason is still unsure

57
Q

All chlorophyll based photosynthesis use what kind of chlorophyll?

A

Chlorophyll A

58
Q

Light absorption of photosynthetic pigments is affected by (2)

A

chemical structure of the chlorophyll
noncovalent interactions of chlorophylls with proteins in photosynthetic membranes

59
Q

Which mineral element is essential to the structure of chlorophyll A?

A

Mg - magnesium

60
Q

What are the four accessory pigments?

A

Chlorophyll B
B carotene
phycoerythrin
phycocyanin
- above 2 are types of phycobilins

61
Q

Why does chlorophyll make plants green?

A

Because it absorbs all colours of light that are not green, so only green is reflected towards our eyes

62
Q
A
63
Q

Function of chromoplasts? (3)

A
  • accessory pigments
  • protecting photosynthesizing organisms from destructive photooxidation - anti oxidants
  • structural roles in assembly of the light harvesting complex
64
Q

What are phycobilins?

A

phycocyanin and phycoerythrin (blue and red)
- linear tetrapyrroles - derived from the same biosynthetic pathway as chlorophyll and heme groups
- water soluble
- accessory pigment with no associated metal

65
Q

What is the only essential pigment?

A

chlorophyll a

66
Q

What are the major factors to sustain plant life from an energy generation standpoint?

A
  • Water - solvent for enzymatic activity and formation of biological membranes
  • air - basic elements C O N H
  • Light - thermonuclear fusion generates ultimate form of energy from the sun which gets passed on to plants
67
Q

What percent of the total energy radiated from the sun is emitted as visible light?

A

~43%

68
Q

What is photosynthetically active/ available radiation?

A

The portion of light that can be captured by autotrophs and used for photosynthesis
- units of umol/m2/sec
- photons / area / time

69
Q

What is the photosynthetic action spectrum?

A
  • magnitude of biological response to light (wavelength)
  • rate of photosynthesis
  • focuses on a single wavelength of light shining on the plant
70
Q

What is the absorption spectrum

A
  • the amount of absorbed light by a molecule (pigment)
  • functional wavelengths of light in photosynthesis
71
Q

Why do pigments capture the light?

A
  • to get energy from photons
  • energy from a photon will excite an electron from the ground state to a higher energy orbital / excited state
72
Q

4 possible photoexcitation outcomes?

A

Heat - thermal dissipation, converting excitation energy to heat - chlorophylls return to ground state

Fluorescence - immidiate reemission of energy as a long wavelength

Energy transfer - excited pigment molecule like chlorophyll transfers its energy to another molecule

Photochemistry - energy of the excited state triggers a chemical reaction and becomes and e donor
- linkage of the excited e donor to a proper e acceptor
- transduction of chemical energy

73
Q

What is the difference between a lower excited state and a higher excited state?

A

some pigments take in higher energy light like blue wavelengths which will excite an electron to a greater degree than a lower energy wavelengths like red light

  • this means that when returning to ground state, an electron in the higher excited state might release heat to return to the lower excited state before returning down to the ground state
74
Q

Explain the energy transfer during photosynthesis?

A
  • purely physical phenomenon
  • no chemical changes
  • done by resonance energy transfer - energy is transferred from pigment to pigment by resonance untll it reaches the reaction center pigment
75
Q

go back and add cards for electron transport and energy transfer lectures (missed two classes)

A
76
Q

4 outcomes of photoexcitation?

A
  1. Heat
    - thermal dissipation - converting excitation energy to heat
    - return to ground state
  2. Fluorescence
    - immediate reemission of energy as a long wavelength
  3. Energy transfer
    - excited pigment molecule transfers its energy to another molecule
  4. Photochemistry
    - energy of the excited state triggers a reaction and becomes an e- donor
    - linkage of e donor to proper e acceptor
    - transduction of chemical energy
77
Q

What is non photochemical quenching? 3 types

A
  • how plants deal with excess light energy
  • losing energy as heat
  1. energy dependent quenching - the xanthophyll cyclce
  2. state transition - conformational changes in light harvesting complex 2
  3. photoinhibition - light induced reaction in quantum yeild as a consequence of damage
78
Q

What is the dominant form of non photochemical quenching and how does it work?

A
  • energy dependent quenching
  • lumen acidification activates VDE
  • zeaxanthin leads to energy dissipation by rearrangement of LCHII and reaction center II - decrease of energy transfer to RCII
  • the structural changes result in dissipation of light enrgy as heat
79
Q

explain the state transition quenching pathway

A

high light intensity will cause accumulation of PQH2
- will activate LHCII kinase - will phosphorylate substrate
- phosphorylated LHCII prevents energy transfer to PSII

80
Q

explain the photoinhibiton quenching pathway

A

D1 protein of PSII is succeptible to photodamage - photosynthesis is inhibited
- OEC is inactivated - followed by reaction center
- will dissolve and digest D1 protein before reforming it and reactivating PSII

81
Q

All forms of quenching target ____________ to some degree to regulate electron transport?

A

photosystem II

82
Q

How do herbicides work?

A

DCMU - targets plastoquinone so it cannot transport electrons from PSII to PSI

Paraquat - prevents reduction of NADP+ by accepting electrons in PSI

83
Q

What is needed to fix carbon in the calvin cycle?

A

3 CO2, 3 ATP, 2 NADPH
- Rubisco enzyme

84
Q

Explain the steps of the calvin cycle

A

Carboxylation - 3X RuBP and 3xCO2 to make 6 3-PGA via RUBISCO
Reduction - 6ATP and 6 NADPH to reduce 3-PGA to make 6 G3P - 1 G3P leaves for energy - 5 G3P regenerate RuBP

85
Q

How does RUBISCO act as a carboxylase and an oxygenase

A
  • carboxylase - takes CO2 and RuBP to make 2 3 PGA
  • oxygenase - takes O2 and RuBP to make 1 3 PGA and 2PG
86
Q

Can rubisco discriminate between CO2 and O2

A

Not well - catalytic domains are too similar

87
Q

What is the usual form of RUBSICO in plants?

A

Form 1 in most plants - hexadecameric form
- 8 large subunits and 8 small subunits
- found in some other forms in algae and bacteria

88
Q

Is RUBISCO efficient? How does this affect plants

A

RUBISCO is very slow and inefficient as it will often bind O2 instead of CO2
- to make up for this, plants will make lots of it - up to 50%

89
Q

How is RUBISCO regulated at the transcription, assembly and inhibition levels?

A
  • transcription of rbcS in nuclear genome, but rbcL gene is transcribed in plastid - chloroplast- genome
  • several chaperone proteins are required to assemble
  • activity is inhibited by a sugar phosphate inhibitor, rubisco activase removes the inhibitor
90
Q

What amino acid is required in the active site of RUBISCO that must be carbamylated for proper activity?

A

A lysine is carbamylated in the active site of rubisco

91
Q

What 4 things are needed to make active rubisco?

A

Carbamylated lysine
- Mg
- CABP
- CA1P - sugar phosphate inhibitor

92
Q

you missed alot of lectures :/

A

yup

93
Q

How do carboxylases compare between C4 and CAM plants

A

C4 - carboxylases are spatially seperated - mesophyll and bundle sheath cells
CAM - carboxylases are temporally seperated - day and night

94
Q

do C4 and CAM plants use Rubisco enzyme for the first carboxylation?

A

No, they uses PEPC, C3 plants use Rubisco

95
Q

Three factors that influence photosynthesis rate?

A

Light
- less photosynthesis in less light
- no effect on the rate of photosynthesis above the optimum condition

CO2 concentration
- decrease of photosynthesis rate in low CO2 concentration
- no effect on the rate of photosynthesis above the optimum

Temperature
- lower photosynthesis above or below the optimum temperature

96
Q

Why is there a difference between sun leaves and shade leaves?

A

Chloroplast movement
- will move to surface in shade leaves, will move to edges in sun leaves

97
Q

How are sun and shade leaves different?

A

Sun
- high rate of photosynthesis
- thick leaves
- high stomatal density
- long pallisade cells
- low ratio of chlorophyll to soluble protein

Shade
- low rate of photosynthesis
- thin leaves
- low stomatal density
- short pallisade cells, more spongy mesophyll cells
- high ratio of chlorophyll / soluble proteins

98
Q

In the light response curve, why does the rate of photosynthesis stop at high light intensities?

A

At the light saturation point, photosynthetic reaction rate is determined by light independant reactions - such as carbon fixation

99
Q

In the light response curve, at low light intensities, the relationship between net photosynthesis and light intensity is linear, why?

A

At low light intensities light is limiting for photosynthesis

100
Q
A