Intro Flashcards
3What is the four step movement of water in plants?
- Water moves from soil into the root
- water moves through the root
- water moves up through the xylem
- water moves from leaf to air
How is water moved through a plant, what does it require?
water is pulled upwards by transpiration from leaves - so water movement requires leaves exposed to air
5 important properties of water
Water is cohesive - sticks to itself
Water is an excellent solvent
Water can dissociate into ions
has high latent heat of vaporization - means it is thermodynamically stable
water has a high tensile strength
Three specific properties of water that make transpiration possible?
cohesion - water sticks to itself
adhesion-water sticks to other substances - cell walls of xylem
surface tension - property of liquid surfaces which allows it to resist an external force due to the cohesive nature of its molecules
Another name for the process of plants bringing water from roots to shoots is called?
Capillary action
What is water potential in plants?
Movement of the potential energy of water - water moves from places of high potential energy to areas of low potential energy
What are the three major factors affecting the water potential in plants?
Concentration- solute potential
Pressure potential
Gravity potential
Why is water potential important?
Because it regulated cell growth, photosynthesis and crop productivity
What is the definition of water potential?
the ability of water molecule to move freely in a solution
Solute potential Ws is defined by what?
Gas constant, temperature in K and solute concentration in mol/L
- also needs the ionization constant if ions existed - ie NaCl
- sugar does not ionize so therefore the constant is 1
- NaCl goes to Na+ and Cl- which makes it 2
therefore the system has an ionization constant of 3
Pressure potential Wp is determined by what?
turgor pressure and tension - turgor pressure increases hydrostatic pressure and tension is a decrease in it.
Gravity potential Wg is determined by what?
water density (constant ish), gravitational constant, height of water above the ground
- results in the unit of pressure
What thermodynamic component of Ww is responsible for water movement?
Osmosis or Ws
solute potential is the same as…
osmotic potential
the solute potential is always ___ when solutes are added to eh system?
reduced because there is a negative sign on the equation
- all water systems have some amount of solutes
Define osmosis
phenomenon of water flowing across a semi permeable membrane from areas of low solute concentration to areas of high solute concentration
- water moves to where the stuff is to balance it out
Water moves from areas of high water potential to areas of low water potential, explain how osmosis works with all the math shit
In a system with pure water and water with surcrose
- gravity constant will be ignored
- the system of pure water will have a Ww of 0
- the system with sucrose will have a negative Ws because of -RTC
- therefore the side with sucrose will have a lower total water potential than the side of just pure water - water moved towards the side with sucrose
What value will change to balance Ws or solute potential?
pressure potential Wp - turgor pressure builds up to balance in cells - or plants use solutes to regulate turgor pressure
What is the purpose of a plant cell wall?
Resistance of the cell to deformation - the wall is rigid to provide structural support and to prevent osmotic lysis by turgor pressure
Water limitation does what to turgor pressure?
lowers turgor pressure
If solute concentration is higher outside the cell than inside the cell, what happens and what is it called?
Water will leave the cell, the cell is plasmolyzed and the inside of the cell is hypotonic and the outside is hypertonic
If solute concentration is higher inside the cell than outside, what happens and what is it called?
water will enter the cell, the cell is turgid and the inside of the cell is hypertonic and the outside is hypotonic
How do water molecules move between plant cell membranes?
Through water specific channels called aquaporins
Why is solute potential negative?
Because water will form hydrogen bonds with the solute meaning the energy of the water molecule is tied up with the dissolved solute - therefore there is less potential energy in the water molecules
A system is in equilibrium when…
the pressure potential is the inverse of the solute potential
Wp = -Ws
If the solute potential inside the cell is greater than the solute potential outside the cell (i.e, a smaller negative number) what happens to the cell?
the cell is plasmolyzed and water leaves the cell
- greater solute potential means less solute, water moves to where there is more solute - so would move out of the cell
What is the structure of an aquaporin?
tetrameric arrangement - each monomer forms a water channel - so four channels per aquaporin
- Has an NPA structure - asparagine - proline - alanine
- Asparagine has an NH4+ structure and the slight positive change can pull on the slight negative charge on water
What two things regulate aquaporin channel activity?
phosphorylation and ph control
Under standard conditions aquaporin protein is phosphorylated adn active
under drought conditions aquaporin protein is dephosphorylated and inactive
Under high water conditions the pH drops which will close the aquaporin
All change in water potential begins with ____
surface tension
Define transpiration
evaporation of water mainly through the stomata of leaves
Plant cells become turgid when you put them in pure water. This is because the water potential in the plant cells is lower than the pure water. Is this correct or incorrect?
Yes, water always moves from areas of high water potential to areas of low water potential.
Solutes are in plant cells but not in water, higher solute means a more negative solute potential - brings the water potential down.
An increase in solute concentration means a _____ in solute potential
decrease
How do you effectively stop osmosis?
with pressure - the pressure potential will always balance the solute potential.
What is Ficks law?
describes the movement of water by diffusion
dm/dt = -D A (dc/dx)
dm/dt = amount of substance moved per unit time
D = diffusion coefficient
A = area where flow passes through
dc/dx = gradient of concentration (change in concentration per unit distance in a direction perpendicular to the plane A)
Which thermodynamic component does a plant cell use to drive water movement?
solute potential somehow
How do plants create the thermodynamic gradient that drives water movement through the plant?
transpiration
Which type of mesophyll cells is important for driving water movement
spongy mesophyll cells because they have air gaps that help drive transpiration
Diffusion is faster according to Ficks law when -
the concentration gradient (dc/dx) is steeper
the area (A) is larger
the distance (x) is smaller
D is larger
What are the top and bottom sides of the leaf called?
Adaxial - top
Abaxial - bottom
explain the relationship between evaporation and condensation in relation to the stoma being open or closed
when the stoma is closed evaportation and condensation are equal
- but when the stoma is open the surface tension is increased (?) and evaporation is greater than condensation so there is a net loss of water from the system
structural sugars in the cell wall provide sites for _________ to water molecules
hydrogen bonding sites
4 steps to generating transpirational pull in leaves
- water vapor diffuses from the air space of the leaf to air outside through stomata in transpiration
- the water vapor lost by transpiration is replaced by evaporation from the water film that coats mesophyll cells
- water vapor diffuses from the air space of the leaf to air outside through stomata in transpiration
- evaporation causes the air water interface to retreat further into the cell wall and become more curved as the rate of transpiration increases – diagram with the circles
what is the relationship between the radius of the curvature of the air water interface and the resulting hydrostatic pressure?
As the radius of the curvature decreases, so does the hydrostatic pressure - gets more negative
- infer that greater curvature is greater displacement (bouncier trampoline) so therefore the force generated is stronger
- decreased hydrostatic pressure means increased surface tension
Evaporation results in the air water interface to recede towards outer cellulose microfibrils of the cell wall and then _________ (POLLEV)
Surface tension can be built up at the air water interface
what is the gradient of water potential throughout the plant system?
high water potential is closer to the xylem and low water potential close to the stomata
Why is there less water vapor / evaporation close to the xylem
because there is less airspace
____ evaporation = ____ surface tension = _____ hydrostatic pressure
more evaporation = more surface tension = less hydrostatic pressure
3 pathways for water flow
Apoplasmic pathway - water moves through the intercellular spaces - cell walls - no entry into cells
Symplasmic pathway - water moves into cells via plasmodesmata
Transcellular pathway - water moves across plasma membranes - both between cells and in central vacuole
Three metrics that determine amount of water flow in each pathway
- magnitude of water potential gradient
- tissue capacitance
- resistance to water flow (hydraulic resistance)
7 step process for water movement through the leaf?
- stomata open
- water vapor diffuses from internal air space, down concentration gradient, into the air
- net loss of water vapor causes air water interface to recede towards outer microfibrils
4.establishment of the water potential gradient begins when the air water interface touches outer microfibrils - H bonding / cohesion transmits tension at surface to bulk water movement
- time dependent buildup of surface tension
- water pulled towards air water interface
Which pathway for water movement has basically 0 hydraulic resistance?
apoplasmic because water does not have to cross a membrane
define plasmodesmata
microchannel connecting between plant cells through the cell wall
In plant leaves, can the water potential gradient be developed between xylem and spongy mesophyll cells due to different evaporation rates?
yes it can - evaporation rates are higher by the stomata than at the xylem because of the inter air spaces
How do plants create the thermodynamic gradient that drives water movement through plant - what is the rate of water loss equal to?
evaporation rate - rate of water loss from air water interface = rate of water delivery pulled from interior sources
are xylem cells dead?
mature xylem is dead but immature xylem is alive
explain tracheids
- primitive water conducting elements
- present in gymnosperms and angiosperms
- cell size is species dependent
- consists of long thin walls with tapered ends
- ## reinforced with lignin
tracheids have a ____ _____ that allows for a low resistance pathway to be developed between neighboring tracheids
pit pair
Explain vessels
- advanced water conducting elements
- present only in angiosperms
- one vessel contains n vessel elements
- contain a perforation plate for bulk water flow
- ## forms effectively a longer and wider tube than the tracheids
are wider water transport vessels better than narrower ones?
wider is more efficient at transporting more water and taking up less area - but not good under water stress and prone to xylem embolisms
Define cavitation
a condition where an air bubble moves into a vessel
Define an embolism
the blocking of a xylem vessel or tracheid by an air bubble or cavity
Why might it be important to have vessels and trachieds when discussing embolisms
while vessels can move significantly more water, embolisms can spread between vessels whereas they are confined to single celled trachieds