SDS Page Flashcards
1
Q
SDS
A
Sodium dodecyl sulfate
2
Q
PAGE
A
Polyacrylamide gel electrophoresis
3
Q
Main Function of SDS
A
Isolate target protein
4
Q
Protein Characteristics consideration
A
- Protein Interaction
- Charge
- Molecular Weight
5
Q
Other functions of SDS
A
- eliminate protein interactions
- unfold into rods
- impart negative charge
6
Q
Location of heavier and lighter proteins in the gel
A
heavy proteins - high
light proteins - low
7
Q
Characteristics of SDS
A
2 polymers
Neurotoxic and carcinogenic
Chemically inert
8
Q
Required 2 polymerization agent
A
APS
TEMED
9
Q
Compose of 2 gel
A
Stacking and Resolving gel
10
Q
Lower pH and acrylomide, and larger pores
A
Stacking Gel
11
Q
Higher pH and acrylomide, and smaller pores
A
Resolving Gel
12
Q
Sample treatments
A
Coomassie Brillant Blue R-250 Gel
Glycerol
Dithiothreitol
Bromophenol Blue
