Isolation of Proteins and Bradford Assay

Question 1

Considerations for Blood Protein Isolation

Answer 1

Specificity
Organism Type
Source
Protein location

Question 2

Fraction of blood

Answer 2

Plasma and RBC

Question 3

Process to fraction blood

Answer 3

Centrifuge

Question 4

To prepare plasma for use.

Answer 4

Dilute

Question 5

To prepare red blood cells for use

Answer 5

Cell Disruption by Osmotic and Detergent lysis

Question 6

Used to develop osmotic and detergent lysis

Answer 6

KCl and SDS

Question 7

Another name of Bradford assay

Answer 7

Coomassie Brillant Blue G-250 dye

Question 8

Primary color of Bradford assay

Answer 8

Greenish brown color

Question 9

Relationship of Protein concentration and Bradford Color

Answer 9

Increase concentration and more blue

Question 10

Used to measure color change

Answer 10

Spectrophotometry

Question 11

Tool used to measure spectrophotometry capable of emitting light 190 - 1100 nm

Answer 11

UV-VIS/NIR Spectrophotometer

Question 12

Used to determine the absorption of the unknown protein

Answer 12

Standard curve of known protein