SCN Flashcards

1
Q

Evans (2016)

A

SCN = master clock. Self-sustained rhythms generated by 24h transcriptional-translational feedback loop (clock genes - molecular level). Coordinate rhythms in physiology and behaviour. Synced at tissue level via intercellular signalling (VIP).

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2
Q

Antle (2009)

A

Only some SCN endogenously rhythmic - intercellular signalling with neuropeptides e.g. NPY, also GABA. Possible pharmacological targets for circadian / sleep dysfunction?

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3
Q

Herzog 1997

A

SCN cells still generate rhythms when isolated

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4
Q

Prosser 2007

A

PK2 important signalling factor: syncs core SCN cells to shell. Peaks in day and suppresses locomotor activity in mice

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5
Q

SCN outputs

A

Other hypothalamic areas e.g. aVPN (melatonin release from pineal, CRH release), subventricular zone, DM nucleus, medial preoptic nucleus (GnRH)

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6
Q

DeCoursey 1989

A

SCN ablation and transplant of foetal tissue

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7
Q

DeCoursey 2000

A

Chipmunks

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8
Q

Lucas 2001

A

Blind people still entrain due to melanopsin RGCs

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9
Q

Hattar 2002

A

melanopsin tau:lacZ mouse and retrograde staining from SCN - down RHT to retina (1/100 RGCs)

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10
Q

Lucas 2003

A

Melanopsin RGCs intrinsically photoreceptive, emit APs in vitro. But cells from melanopsin K/O mice don’t show this

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11
Q

Melyan 2005

A

Put melanopsin into non-photoreceptive cell = becomes photoreceptive

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12
Q

Cholera toxin labelling

A

Stain RHT input to SCN: see tracts synapse in ventral pattern

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13
Q

Electromicrographs

A

Postsynaptic densities (RHT synapses) are Gray type I excitatory cells

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14
Q

PACAP immunostaining

A

Ventral pattern of PACAP staining in SCN: closely matches that of RHT innervation

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15
Q

Khalsa 2003

A

Similar pattern of PRC seen in humans e.g. light early in night = phase delay

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16
Q

van del pol 1998

A

Brief pulses (rather than continuous) of light = integrate and can cause even larger phase shifts

17
Q

Piggins and Guilding 2011

A

Photic and non-photic stimuli = differential PRCs

18
Q

Marston 2008

A

Rodents and arousal-promoting stimuli e.g. dark pulse late in subjective day = phase advance. 6h dark promotes arousal = less cFos expressed in SCN, higher activity in orexin neurons

19
Q

Harmar 2002

A

VPAC2R K/O mice = arrythmic in constant dark, no sync of SCN cells. Amp and synchrony of SCN cells impaired - need VIP signalling to synchronise

20
Q

Hughes 2015

A

VIP K/O mice in constant light = improved behavioural rhythms so shows light is crucial

21
Q

Yamaguchi 2013

A

AVPR K/O mice = resistant to jet lag. Entrain/phase shift much more rapidly, behavioural and molecular (clock gene expression)

22
Q

Mieda 2015

A

Also resistant to jet lag if no BMAL in AVP neurons. AVP tries to keep intact clock by resisting large changes in rhythm

23
Q

Piggins and Guilding 2011

A

Action of NTs e.g. Glu and PACAP activate VIP receptors in SCN to impact clock gene expression and set/reset phase

24
Q

Davis 2001

A

Higher breast cancer rates in night shift workers (constantly phase shifting / clock out of sync). Circadian dysfunction can lead to disease

25
Q

van Someren 1997

A

BLT in care homes increases amp of circadian rhythms / improved entrainment (also helps with cognitive problems)

26
Q

Lewy

A

Phase delay hypothesis for SAD

27
Q

Murray 2003

A

Association between lowered/depressed mood in winter and winter phase delay

28
Q

Mistlberger 2005

A

Opponent-process model.
Lesion SCN = homeostatic only, short bouts of sleep/wake.
Ignore sleep homeostat e.g. sleep deprivation = more tired in first night than third day
Forced desynchrony - 28h day. Homeostasis always kept same but measured temp as output of circ clock = 24.3h, freerunning

29
Q

VLPO

A

Light info travels down RHT to VLPO, forms excitatory synapses (can be seen with melanopsin tau:lacZ staining).
VLPO is sleep-promoting (GABAergic neurons that switch off ARAS) so in mice = active during day (higher cFos expression). Lesion VLPO = disrupted sleep.

30
Q

Roenneberg 2009

A

Chronotype: preferred phase of sleep/wake. Males later, changes with age etc. Social schedule interferes considerably with sleep/activity times so should try and change work/school schedules to suit chronotype!
Social jet lag = wake up too early on weekdays, sleep deprived. Worse in young adults.
Circadian clock controls sleep but according to solar cycle not social schedule!

31
Q

LeGates 2014

A

Irregular light linked with depression.