SCN Flashcards
Evans (2016)
SCN = master clock. Self-sustained rhythms generated by 24h transcriptional-translational feedback loop (clock genes - molecular level). Coordinate rhythms in physiology and behaviour. Synced at tissue level via intercellular signalling (VIP).
Antle (2009)
Only some SCN endogenously rhythmic - intercellular signalling with neuropeptides e.g. NPY, also GABA. Possible pharmacological targets for circadian / sleep dysfunction?
Herzog 1997
SCN cells still generate rhythms when isolated
Prosser 2007
PK2 important signalling factor: syncs core SCN cells to shell. Peaks in day and suppresses locomotor activity in mice
SCN outputs
Other hypothalamic areas e.g. aVPN (melatonin release from pineal, CRH release), subventricular zone, DM nucleus, medial preoptic nucleus (GnRH)
DeCoursey 1989
SCN ablation and transplant of foetal tissue
DeCoursey 2000
Chipmunks
Lucas 2001
Blind people still entrain due to melanopsin RGCs
Hattar 2002
melanopsin tau:lacZ mouse and retrograde staining from SCN - down RHT to retina (1/100 RGCs)
Lucas 2003
Melanopsin RGCs intrinsically photoreceptive, emit APs in vitro. But cells from melanopsin K/O mice don’t show this
Melyan 2005
Put melanopsin into non-photoreceptive cell = becomes photoreceptive
Cholera toxin labelling
Stain RHT input to SCN: see tracts synapse in ventral pattern
Electromicrographs
Postsynaptic densities (RHT synapses) are Gray type I excitatory cells
PACAP immunostaining
Ventral pattern of PACAP staining in SCN: closely matches that of RHT innervation
Khalsa 2003
Similar pattern of PRC seen in humans e.g. light early in night = phase delay