SB1 Flashcards

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1
Q

What do cells make up?

A

All living things

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2
Q

Name the two types of cell?

A

Eukaryotic

Prokaryotic

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3
Q

What are eukaryotic cells?

A

They’re complex and include all animal and plant cells.

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4
Q

What are prokaryotic cells?

A

They’re smaller and simpler than eukaryotic cells (e.g - bacteria)

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5
Q

What are Eukaryotes?

A

They’re organisms that are made up of eukaryotic cells.

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6
Q

What are prokaryotes?

A

They’re prokaryotic cells. (Single-called organisms).

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7
Q

What sub-cellular structures do most animal cells have?

A
  1. Nucleus - contains genetic material that controls the activities of the cell. Genetic material is arranged into chromosomes.
  2. Cytoplasm - gel-like substance where most of the chemical reactions happen. It contains enzymes that control these reactions.
  3. Cell membrane - holds the cell together and controls what goes in and out.
  4. Mitochondria - these are where most of the reactions fro respiration take place. Respiration transfers energy that the cell need to work.
  5. Ribosomes - these are involved in the translation of genetic material in the synthesis of proteins.
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8
Q

What sub-cellular structures do most plant cells have?

A

They usually have all the bits the animal cells have plus extra :

  1. Nucleus 2. Cytoplasm 3. Cell membrane 4. Mitochondria 5. Ribosomes
  2. Cell wall - made of cellulose. It supports the cell and strengthens it.
  3. Large vacuole - contains cell sap, a weak solution of sugar and salts. It maintains the internal pressure to support the cell.
  4. Chloroplasts - these are where photosynthesis occurs, which makes food for the plant. They contain a green substance called chlorophyll.
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9
Q

What sub-cellular structures do most bacterial cells have?

A
  1. Chromosomal DNA - controls the cells activities and replication. It floats free in the cytoplasm.
  2. Ribosomes 3. Cell membrane
  3. Plasmid DNA - small lops of extra DNA that aren’t part of the chromosome. Plasmids contain genes for things like drug resistance, and can be passed between bacteria.
  4. Flagellum - a long hair-like structure that rotates to make the bacterium move. It can be used to move the bacteria away from harmful substances like toxins and towards beneficial things like nutrients or oxygen.
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10
Q

What’s the difference between bacterial cells and plant + animal cells?

A

Bacterial cells are a lot smaller and contain chromosomal DNA, flagellum and plasmid DNA.

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11
Q

What are multicellular organisms?

A

They contain lots of different types of cells (cells with different structures).

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12
Q

What are specialised cells?

A

They’re cells that have a structure which makes them adapted to their function.

E.g - egg, sperm and ciliated cells.

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13
Q

How is an egg cell adapted to its function?

A

The main functions of an egg are to carry the female DNA and to nourish the developing embryo in the early stages.

  1. It contains nutrients in the cytoplasm to feed the embryo.
  2. It has a haploid nucleus.
  3. Straight after fertilisation, its membrane changes structure to stop any more sperm getting in. This makes sure the offspring end up with the right amount of DNA.
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14
Q

How is a sperm cell adapted to its function?

A

The function of a sperm is to transport the males DNA to the females egg.

  1. A sperm cell has a long tail so it can swim to the egg.
  2. It has lots of mitochondria in the middle section to provide the energy (from respiration) needed to swim this distance.
  3. It also has an acrosome at the front of the ‘head’, where it stores enzymes needed to digest its way through the membrane of an egg cell.
  4. It also contains a haploid nucleus.
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15
Q

How is a ciliated epithelial cell adapted to its function?

A

They’re specialised for moving materials.

  1. Epithelial cells line the surfaces of organs.
  2. Some of them have cilia (hair-like structures) on the top surface of the cell.
  3. The cilia beta to move substances in one direction, along the surface of the tissue.
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16
Q

What’s the calculation for magnification?

A

Magnification = image size
—————
Real size

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17
Q

What are enzymes?

A

They’re catalysts produced by living things.

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18
Q

What is an active site?

A

Every enzyme has an active site.

The part where it joins on to its substrate to catalyse the reaction.

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19
Q

What’s the substrate?

A

It’s the molecule changed in the reaction.

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20
Q

Explain how enzymes work with substrates?

A

They usually only work with one substrate. They’re said to have high specificity for their substrate.

This is because, for the enzyme to work, the substrate has to fit into the active site. If the substrates shape doesn’t match the active sites shape, then the reaction wont be catalysed. This is called the lock and key mechanism, because the substrate fits into the enzyme just like a key fits into a lock.

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21
Q

What affects the rate of reaction?

A

Temperature, pH, substrate concentration.

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22
Q

How does changing the temperature, change the rate of an enzyme-catalyst reaction?

A
  1. A higher temperature increase the rate at first. But if it gets too hot, some of the bonds holding the enzyme together break. This changes the shape of the enzymes active site, so the substrate won’t fit any more.

The enzyme is said to have been denatured.

  1. All enzymes have an optimum temperature that they work bets at.
23
Q

How does changing the pH, change the rate of an enzyme-catalyst reaction?

A
  1. If it’s too high or low, the pH interferes with the bonds holding the enzymes together. This changes the shape of the active site and dentures the enzymes.
  2. All enzymes have an optimum pH that they work best at. It’s often neutral pH 7, but not always.
24
Q

How does changing the substrate concentration, change the rate of an enzyme-catalyst reaction?

A
  1. The higher the concentration, the faster the reaction. This is because its more likely that the enzyme will meet up and react with a substrate molecule.
  2. This is only true up to a certain point. After that, there are so many substrate molecules that the enzymes have about as much as they can cope with, so adding makes no difference.
25
Q

How do you calculate the rate of reaction?

A

Rate = 1000
———
Time

26
Q

Why is it important for organisms to be able to break down big molecules?

A

Proteins, lipids and some carbohydrates are big molecules.

They need to be broken down into their smaller components so they can be used for growth and other life processes.

27
Q

explain what enzymes break down the big molecules and what they get broken down into

A
  1. Carbohydrates - amylase = simple sugars
  2. Proteins - protease = amino acids
  3. Lipids - lipase = glycerol and fatty acids
28
Q

How can you test for sugars?

A

Using Benedicts solution.

29
Q

What happens in the Benedicts test?

A
  1. Add Benedicts reagent to a sample and heat it in a water bath that’s set to 75 degrees.
    If the test is positive it will form a coloured precipitate
30
Q

What does each colour represent using Benedicts solution?

A
Blue = none 
Green = some
Yellow = bit more 
Orange = high
Brick red = very high
31
Q

How is starch tested?

A

Using iodine

32
Q

What happens if starch is present?

A

Just add iodine solution to the test sample.

  1. If starch is present, the sample changes from browny-orange to a dark, blue-black colour.
  2. If there’s no starch, it stays browny-orange.
33
Q

What test is used for lipids

A

The emulsion test.

34
Q

How does the emulsion test work?

A
  1. Shake the test substance with ethanol for about a minute until it dissolves, then pour the solution into water.
  2. If there are any lipids present, they will precipitate out of the liquid and show up as a milky emulsion.
  3. The more lipid there is, the more noticeable the milky colour will be.
35
Q

What test is used for proteins?

A

Biuret test

36
Q

How does the biruet test work?

A
  1. Add a few drops of potassium hydroxide solution to make the solution alkaline.
  2. Then add some copper sulphate solution - if there’s no protein, the solution stays blue but if its present, the solution will turn purple.
37
Q

What’s diffusion?

A

It’s the gradual movement of particles from places where there are lots of them to places where there are fewer of them.

Or

Diffusion is the net (overall) movement of particles from an area of higher concentration to an area of lower concentration.

38
Q

What is osmosis?

A

Osmosis is the net movement of water molecules across a partially permeable membrane from a region of higher water concentration to a region of lower water concentration.

39
Q

What is active transport?

A

Is the movement of particles across a membrane against a concentration gradient (from an area of low concentration to higher concentration) using energy transferred during respiration.

40
Q

How do you calculate the energy in food?

A

Energy in food = mass in water X temperature change of water X 4.2

41
Q

What’s the method for experimenting osmosis?

A
  1. Prepare sucrose solutions of different concentrations ranging from pure water to a very concentrated sucrose solution.
  2. Use a cork borer to cut a potato into the same sized pieces (preferably from the same potato)
  3. Measure the mass for each chip.
  4. Place one chip in each solution.
  5. Leave the chips in the solution for at least 40 mins.
  6. Remove the chips and pat dry gently with a paper towel. This remove any excess water from the surface of the chip, so you get a more accurate measurement.
  7. Weight the chips again and record the results.
42
Q

How do you find the percentage change?

A

Final mass - initial mass / initial mass X 100.

43
Q

What is a nucleus?

A

Contains genetic material that controls the activities of the cell.

Genetic material is arranged into chromosomes.

44
Q

What is cytoplasm?

A

Gel-like substance where most of the chemical reactions happen.

It contains enzymes that control the chemical reactions.

45
Q

What is a cell membrane?

A

Holds the cell together and controls what goes in and out.

46
Q

What are mitochondria?

A

These are where most of the reactions for respiration take place.

Respiration transfers energy that the cell needs to work.

47
Q

What are ribosomes?

A

Theses are involved in translation of genetic material in the synthesis of proteins.

48
Q

What is a cell wall?

A

Made of cellulose.

It supports the cell and strengthens it.

49
Q

What is a vacuole?

A

Contains cell sap, a weak solution of sugar and salts.

It maintains the internal pressure to support the cell.

50
Q

What are chloroplasts?

A

These are where photosynthesis occurs, which makes food for the plant.

They contain a green substance called chlorophyll.

51
Q

What is chromosomal DNA?

A

One long circular chromosome.

Controls the cells activities and replication.
It floats free in the cytoplasm (not in a nucleus).

52
Q

What is plasmid DNA?

A

Small loops of extra DNA that aren’t part of the chromosome.

Plasmids contain genes for things like drug resistance, and can be passed between bacteria.

53
Q

What is a flagellum?

A

A long, hair-like structure that rotates to make bacterium move.

It can be sued to move the bacteria away from harmful substances like toxins and towards beneficial things like nutrients or oxygen.