Santangelo - intro

Question 1

What are the Rag proteins?

Answer 1

They are the proteins that create the double stranded break in the DNA within the VDJ segments.

Question 2

What are the precursors for the immune system cells and where does each come from?

Answer 2

It all starts with stem cells. From There they can become either myeloid or lymphoid cells.. From there, the lymphoid cells become members of the adaptive immune system. The myeloid cells become members of the innate immune system.

Question 3

What cells have CD25?

Answer 3

Treg

Question 4

What CD will all T cells have?

Answer 4

CD3

Question 5

Humoral immunity vs. cell mediated immunity

Answer 5

Humoral === B. Cells

Cell-mediated = T cells

Question 6

Adjuvant

Answer 6

An agent that stimulates the immune system and, for example, increases the responsiveness to the vaccine. But, it does not have a specific antigenic affect on itself.
- for example, antigens might not actually cause a response. Sometimes you have to mix it with an adjuvant to make an immune response occur.

Question 7

Flow. Cytometry

Answer 7

A way to look at multiple protein expression on a cell. - You have one cell at a time going through a nozzle. It then hits laser beams which is read and depicted as a color.

Question 8

CBC

Answer 8

Done through flow Cytometry. It shows the different population of cell based on size

Question 9

FACS - Fluorescence Activated Cell Sorting

Answer 9

Using fluorescent take in order to understand which cell surface receptors are being expressed on various cells. We can also tell how much of each specific protein is being expressed.

Question 10

Hyper variable vs framework region

Answer 10

Pretty much all antibodies are the same in the variable region in most places. The only places that are different are in areas called the “hyper variable” region. The framework region is the region that largely stays the same.