Salmonella enteritidis

Question 1

gram stain

Answer 1

gram negative rod

Question 2

Media

Answer 2

grown on MacConkey, HE, XLD, SS, Selenite

Question 3

Appearance on media

Answer 3

NLF: clear on all three media, blanck centers for most species on HE and XLD

Question 4

Mimicking

Answer 4

citrobacter, differentiated by lactose

Question 5

IMViC

Answer 5

• • • +/- (species identified by serologically and/or biochemically)

Question 6

Indole

Answer 6

negative

Question 7

Methyl red

Answer 7

positive

Question 8

Voges Proskauer

Answer 8

negative

Question 9

Citrate

Answer 9

positive or negative is variable

Question 10

Lactose fermenting

Answer 10

negative

Question 11

Motility

Answer 11

positive

Question 12

H2S production

Answer 12

positive (most species)

Question 13

Oxidase

Answer 13

negative

Question 14

TSI

Answer 14

K/A H2S +

Question 15

LIA

Answer 15

K/K H2S+

Question 16

Diseases

Answer 16

gastroenteritis, produces severe diarrhea, vomiting and fever in 10-28 hours, cures spontaneously after 2/7 days; bacteremia/extraintestinal infection (blood stream infection); enteric fever (typhoid fever); paratyphoid fever: less severe than typhoid fever, bacteremia, often mistake for Fever of Unknown Origin

Question 17

Transmission

Answer 17

contaminated food (eggs, meat, mayonnaise, etc…); person to person via fecal-oral route by ingestion of contaminated food or water; healthcare settings when handwashing guidelines are not followed.

Question 18

Reservoirs

Answer 18

not part of normal bowel flora; carrier states may exist in stools

Question 19

Vectors

Answer 19

farm animals, reptiles, pet turtles, fowl, and various animals

Question 20

Serotyping

Answer 20

Kauffman-White scheme

Salmonella O Ag -grouping A-I and Vi

Question 21

Endemic areas

Answer 21

2-4 million cases in US per year

Question 22

Specimen

Answer 22

blood