SAFETY IN IMMUNO Flashcards
A non-profit educational organization that provides a forum for fhe development, promotion, and use of national and international standards
Clinical and Laboratory Standard Institute
Part of the US Department of Health and Human Services Public Health
Centers for Disease and Prevention
General safety practices
- staff must wear lab coats and be additionally protected from contamination by infectious agents
- foods and drinks should not be consumed at work areas or stored in the same area as specimens. Containers, refrigerators, freezers should be marked as containing a biohazard
- specimens needing centrifugation are capped and placed inti a centrifuge with a sealed dome
- a gauze square is used when opening rubber-stopper test tube to minimize aerosol production
- autodilutors or safety bulbs are used for pipetting. Pipetting of any clinical material by mouth is strictly forbidden.
Instituded by CDC in 1985 to protect healthcare workers from exposure to bloodborne pathogens, primarily hepatitis B virus and HIV
Universal Precautions
Enumerate UP
- wearing gloves when collecting or handling blood or any body fluids contaminated with blood
- wearing face shield when there is danger of blood splashing on mucous membrane
- disposing all needles and sharp objects in puncture resistant containers without recapping
Not limited to bloodborne pathogens anf considers all body fluid and moist body susbtances to be potentially infectious
Body substance isolation
Assumes every person in tge healthcare setting is potentially infected or colonized by an organism that could be transmitted
Standard precaution
What is the disadvantage of BSI
- does not recommend handwashing after removing gloves unless visual contamination is present
What is the second level?
Isolation precautions/transmission-based precautions
What is the first level of precaution
Standard precautions
Specifies precautions to use in caring for all patients regardless of diagnosis or presumed infection status.
Standard precautions
Specifies precautions to use for patients either suspected or known to be infected with certain pathogens transmitted by airborne, droplet, or contact routes.
Isolation precaution
used for patients known or suspected to be infected or colonizedwithhighly transmissible or epidemiologically significant pathogens that require special precautions in addition to standard precautions.
TRANSMISSION-BASED PRECAUTIONS/ISOLATION PRECAUTIONS
These must be used in addition to standard precautions for patients known or suspected to be infected with microorganisms transmitted by airborne droplet nuclei (particles smaller than 5m)
Airborne precautions
Itisgeneratedwhenapatienttalks, coughs, or sneezes and during certain procedures such as suctioning
Droplet precautions
These must be used in addition to standard precautions when a patient is known or suspected to be infected or colonized with epidemiologically important microorganisms that can be transmitted by direct contact with the patient or indirect contact with surfaces or patient-care items.
Contact precautions
OSHA requires laboratories to have a personal protective equipment (PPE) program.
- selection and use of gloves
- facial barrier protection and occlusive bandages
- lab gown as barrier protection
Hands should always be washed at the following times:
- Beforepatientcontact,when gloves are removed
- Priortoleavingtheworkarea
- Whenever the hands have been
knowingly contaminated - Beforegoingtodesignatedbreak areas, and before and after using
bathroom facilities