C-REACTIVE

Question 1

principle of C-reactive protein

Answer 1

Reverse Passive Agglutination

Question 2

PRE-ANALYTICAL PHASE

Answer 2

• The work area of the experiment should be sterilized
• Students should wear proper PPE before the experiment
• Standard precaution is applied to every phase of
  experiment

Question 3

Contains polystyrene latex
particles coated with antihuman CRP in a stabilized buffer with less than 0.1%
sodium azide preservative.

Answer 3

CRP Latex Reagent

Question 4

Human serum that has been
diluted and stabilized with
buffer and contain less than
0.1% sodium azide as
preservative.

Answer 4

CRP negative control

Question 5

Human Serum that contains
more than 6 mg/L CRP and
CRP Positive Control less than 0.1% sodium azide
as preservative

Answer 5

CRP positive control

Question 6

To be diluted 1:20 with
distilled water.

Answer 6

Glycine-Saline Buffer:
(20x) Concentrate

Question 7

Positive reaction of CRP

Answer 7

Agglutination

Question 8

Negative reaction of CRP

Answer 8

Smooth milky suspension

Question 9

is a trace constituent of
serum originally thought to be an antibody to the C-polysaccharide of pneumococci

Answer 9

C-reactive protein

Question 10

Who observed that serum from patients with Streptococcus pneumoniae infection precipitated with a soluble extract of the bacteria.

Answer 10

Tillet and Francis

Question 11

Elevated levels are found in conditions such as ______

Answer 11

bacterial infections, rheumatic fever, viral infections, malignant diseases, tuberculosis, and after a heart
attack

Question 12

most widely used indicator of acute
inflammation

Answer 12

C-reactive protein

Question 13

CRP is a relatively stable serum protein with a half-life of about _______

Answer 13

18 hours

Question 14

CRP is easily destroyed by
heating serum to _____

Answer 14

56°C for 30 minutes.

Question 15

Normal level of CRP concentration

Answer 15

0.47 to 1.34 mg/L

Question 16

CRP concentration of less
than 1 mg/L is associated with a low risk for ____

Answer 16

cardiovascular disease

Question 17

1 to 3 mg/L is associated
with an _____

Answer 17

average risk

Question 18

greater than 3 mg/L is
associated with a ____

Answer 18

high risk

Question 19

Normal levels in adults range from
approximately

Answer 19

0.47 to 1.34 mg/L

Question 20

A mean for people with no coronary artery disease is

Answer 20

0.87 mg/L

Question 21

When do we perform serial dilution?

Answer 21

if there’s prozone effect or antigen excess

Question 22

How to dispose preservative sodium azide?

Answer 22

flush with a large volume of water to prevent metal azide build up

Question 23

Proper storage of reagent

Answer 23

store reagents and controls in 2-8
degrees celcius. DO NOT FREEZE