RVT - Protazoa Flashcards
What phyla do Cystoisospora, Toxoplasma gondii and Cryptosporidium belong to?
Apicomplexa
What phyla does Balantidium coli belong to?
Ciliophora
What is the common name for Cystoisospora?
Coccidia
What coccidia will we typically find in dogs?
Isospora canis
Isospora ohioensis
What cystoisospora will we typically find in cats?
Isospora felis
Isospora rivolta
What is the PPP for isospora?
1 week
What are the clinical signs of a coccidia infection?
Watery diarrhea sometimes with blood.
Puppies and kittens can become dehydrated and die
What is the mode of infection for coccidia?
Dog or cat ingests sporulated oocyst. 8 sporozoites are released and invade intestinal epithelium.
What phyla do Giardia and Tritrichomonas belong to?
Sarcomastigorophora
What condition is Toxoplasma gondii linked to in humans?
Recklessness/Inhibition
Where is Toxoplasma gondii found in its definitive host?
Small intestines of the cat
What is the intermediate host of Toxoplasma gondii?
Many warm blooded vertebrates
What are 4 ways a human could be infected by Toxoplasma gondii?
Fecal / Oral
Undercooked meat
Transplacentally
Transmammary ( questionable)
What is the suggested treatment for Toxoplasma gondii?
Clindamycin
How is T. gondii diagnosed?
PCR
IgG and Igm titres
Note- Vial and centrifugal methods not reliable
How many days before Toxoplasma gondii oocysts become infective?
1-5 days
What is the leading cause for abortion in small ruminants?
Toxoplasma gondii
What is the most common definitive host for Crytosporidium?
Calves ( but can also be found in pigs and humans)
Which species of Crytoporidium is zoonotic to humans?
C. felis and C. canis ( they are not seen much)
Where is Cryptosporidium found in its host?
Small intestines
What are signs of Cryptosporidium infection?
Profuse watery diarrhea
What is the life cycle of Crytosporidium?
Oocyst ingested by host, 8 sporozoites released in small intestine. They undergo 2 types of reproduction ( sexual and asexual) The sporulated cyst leave the cells most of which pass out with feces. Some will excyst, invade cells and begin lifecycle again.
How is Cryptosporidium diagnosed?
OVC puddle technique
PCR
Acid fast stain of direct fecal smear
Fecal flotation ( may be hard to see oocysts)
How is Cryptosporidium treated?
None
Symptomatically
There are various genotypes of Giardia. What do assemblages A and B infect?
Humans
Assemblages C and D of Giardia affect what species?
Dogs
What Giardia assemblage affects cats?
F
What Giardia assemblage affects horses, cattle , sheep and goats?
E
Can cats and dogs give Giardia to humans?
No. But humans can give the A assemblage of Giardia to their cats and dogs.
What is the PPP for Giardia?
Like most protazoa, the PPP is quick
1 week
What are clinical signs of a Giardia infection?
Chronic soft stools
Mucus in stool
What is the mode of infection of Giardia?
Ingestion of oocyst
Cyst released trophozoite in small intestine
What protozoan cyst can survive in the environment for an extended period of time?
Giardia
How is Giardia diagnosed?
Direct fecal smear
Elisa
PCR
Centrifugal flotation with sugar solution or zinc sulfate
How is a Giardia infection treated?
Metronidazole
Fenbendazole
What parasite is transmitted by ingestion of infected muscle?
Sarcocystis
What protozoa found in dogs is a common cause of spontaneous abortion in barn animals?
Neospora caninum
What stage of Neospora infection is the rapidly dividing stage?
Tachyozoites
What is the slower dividing stage of Neospora caninum?
Bradyzoites
Tritrichomonus blagburni causes chronic diarrhea in what species?
Cat
When is Tritrichomonus blagburni usually seen in cats and where does it reside in its host?
0-24 months ( young cats and kittens)
Colon and cecum
What is infection with Cystoisospora called?
Coccidia
What are the clinical signs of Coccidian infection?
Watery diarrhea sometimes with blood
How are Cystoisospora diagnosed?
Standard vial or centrifugal flotation
Which protozoan infection is diagnosed using the OVC puddle technique?
Cryptosporidium
What are the clinical signs of Neospora caninum infection in its definitive host?
Puppies can have hind limb paralysis, cervical weakness and dysphagia.
Adult dogs may have CNS signs or polymyositis
How is Neospora caninum diagnosed?
Elisa ( for antibodies)
PCR