RUMBLE ROYAL

Question 1

highlights the details of the organism which help medical technologists in proper identification of bacteria

Answer 1

Staining

Question 2

A test that provides clinician with therapeutic guidelines , indicates which antibiotic is effective in killing the bacteria

Answer 2

Antibiotic Susceptibility Testing

Question 3

Type of stains

• Only one stain is used
• All structures are stained with the same color
• Organism should only be observed for size, shape, and uniformity of staining

Answer 3

Simple stain (Methylene Blue)

Question 4

Type of stains

• Used to distinguish between group of bacteria
• Used to differentiate various type of bacteria that have similar morphologic features

Answer 4

Differential Stain

Question 5

Reagents used in differential stains

Answer 5

Crystal violet = primary stain

Gram’s iodine = mordant

Alcohol – acetone solution – decolorizer

Safranin = counterstain
▪ Gram – positive = purple/ blue stain
▪ Gram – negative = pink/ red stain

Question 6

Types of Antimicrobial Assays

This test is Based on growth inhibition surrounding antibiotic – impregnated disks and yields qualitative results (zone of inhibition)

Answer 6

Disk Diffusion Susceptibility Test (Kirby – Bauer Method)

Question 7

Types of Antimicrobial Assays

Used to determine both the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of a sample bacteria and yields quantitative results

Answer 7

Broth Dilution Susceptibility Test

Question 8

The lowest concentration of antimicrobial agent that inhibits bacterial growth

Answer 8

The lowest concentration of antimicrobial agent that inhibits bacterial growth