RTK and GPCR Flashcards
Which receptor type mediates a variety of growth factors as well as insulin?
RTK
Which growth factor is a monomer?
Epidermal
Which domains bind RTK?
SH2 and PTB
PH domain
Binds lipids, like PIP2 and PIP3
Grb2 domains
SH2-SH3-SH2
Role of SOS
GEF
Order of kinases in MAPK pathway
RAF, MEK, ERK
Mechanisms to inactivate MAPK
- Spontaneous hydrolysis of GTP to GDP inactivates Ras
- RAS-GAP
- protein phosphatase
- Internalization of the receptor
PI3 kinase subunits
p85 with two SH2 domains
p110 with Ras-GTP binding domain
AKT domains
PH and kinase, which is inactive under basal conditions.
Where does PDK phosphorylate AKT?
Threonine residue
Where does TORC2 phosphorylate AKT?
Serine residue
What is TOR?
Ser/Thr protein kinase
What role does mTORC1 do in humans?
Increases cell growth by stimulating protein synthesis
Rheb
Ras homolog which activates mTORC1 when in Rheb-GTP form
TSC1/2
GAP for Rheb
Role of AKT in mTORC1 regulation?
AKT phosphorylates TSC2, which inactivates it so it cannot perform GAP activity for Rheb to inactivate mTORC1
How to treat somatic mutation in TSC1/2
Inhibit mTOR with rapamycin
Termination of PI3K signaling
PTEN - PIP 3’ phosphatase
SHIP2 - PIP 5’ phosphatase
PLC gamma domains and activation
Has two SH2 domains and PH domain. Activated
PLC gamma target
Hydrolyzes PIP2 to form a DAG and IP3
TKAR structure
Pre-formed dimer bound to JAK
STAT
Binds to phosphorylated TKAR and is phosphorylated by JAK
Target of STAT
Transcription factors in nucleus
