DNA replication Flashcards

1
Q

prokaryotic initiator protein

A

DnaA

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2
Q

OriC

A

Single origin of replication in bacteria

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3
Q

Composition of sequence adjacent to OriC

A

AT rich. Base pairing is less stable than GC so DNA can open more easily to start the replication bubble.

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4
Q

DnaB

A

helicase in prokaryotes (DnaC helps)

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5
Q

DnaG

A

primase in prokaryotes

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6
Q

DNA polymerase I function

A

DNA polymerase I function

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7
Q

DNA polymerase III function

A

Replicative chain elongation

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8
Q

Exonuclease activity of DNA pol I

A

5’ to 3’ (unique to pol I) and 3’ to 5’

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9
Q

Which prokaryotic DNA polymerase has higher processivity?

A

DNA polymerase III

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10
Q

Beta subunit of DNA pol III

A

sliding clamp with low internal affinity for DNA. Responsible for high processivity of DNA pol III.

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11
Q

Gamma complex of DNA pol III

A

clamp loader

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12
Q

Core enzyme of DNA pol III

A

Theta, epsilon, and alpha subunits

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13
Q

𝛂 subunit of DNA pol III

A

polymerase

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14
Q

ε subunit of DNA pol III

A

proofreader with 3’ exonuclease ability

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15
Q

ter sites

A

Indicate termination region. Binding site for TUR protein

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16
Q

ek DNA pol for leading strand replication

A

DNA pol 𝛆

17
Q

ek DNA pol for lagging strand replication

A

DNA pol 𝛅

18
Q

ek DNA pol for repair

A

DNA pol β

19
Q

ek DNA pol with high processivity with PCNA

A

DNA pol 𝛅

20
Q

DNA polymerases highly sensitive to aphidicolin

A

alpha, delta, and epsilon

21
Q

PCNA

A

equivalent to the beta subunit of polymerase III–sliding clamp shaped like a ring.

22
Q

Origin of replication in yeast

A

ARS - autonomous replicating sequences

23
Q

Helicase in yeast

A

MCM - mini chromosome maintenance proteins

24
Q

Stabilize ssDNA in yeast

A

RPA - replication protein a

25
Synthesizes DNA in mitochondria
DNA polymerase γ
26
Removes RNA primers in yeast
Rnase H and Fen1 (flap endonuclease 1)
27
What does the pre-replication complex include?
ORC, inactive helicase (MCM), other proteins
28
pk DNA polymerase involved in SOS repair
DNA pol II, IV, and V
29
What does the holoenzyme complex of DNA pol III consist of?
2x (α, ε, θ, β, and τ) and 1x (δ, δ’, γ, χ and ψ)
30
Which ek DNA polymerases lack 3' exonuclease activity?
Alpha and beta. These cannot proofread.
31
What kind of topoisomerase separates interlinking strand of replicated DNA in prokaryotes.
Topoisomerase type II
32
Licensing
Assembly of pre-replication complex
33
Anticipation
Worsening of a disease with successive generations
34
Protein component of telomerase
hTERT - human telomerase reverse transcriptase
35
RNA component of telomerase
hTR - human telomerase RNA
36
How is the 3' overhang stabilized?
Loop structure with proteins called TTAGGG repeat binding factors (TRF-1 and TRF-2)