RR2

Question 1

What are the three stages of transcription?

Answer 1

• Initiation (rate limiting step)
• Elongation
• Transcription

Question 2

What is polycistronic transcription?

Answer 2

Prokaryotic transcription
- Has multiple translation start sites
- Translated by RNAs into bacteria
- RNA polymerase can make multiple gene plots

Question 3

What is monocistronic transcription?
How many RNA polymerases does it have?

Answer 3

Eukaryotic transcription
- Single translational start site
- 3 RNA polymerases

Question 4

What are the different sensitivites of the three RNA polymerases to alpha amanitin?

Answer 4

• Pol I is not impacted
• Pol III runs well at low concentrations
• Pol II doesn’t run at all

Question 5

Name two main traits of RNA pol I , and what parts of it are pretranscribed?

Answer 5

• creates ribosome components
• Has role in protein synthesis

pre-rRNA is pretranscribed

Question 6

What parts of RNA pol II are pre-transcribed?

Answer 6

mRNAs
snRNAs
siRNAs
miRNAs

Question 7

What are 3 main functions of RNA pol II?

Answer 7

• encodes protein
• RNA splicing
• Chromatin-mediated repression - translational control

Question 8

What is pretranscribed in RNA pol III?

Answer 8

• tRNAs
• 5s RNAs
• snRNA U6
• 7S RNA

Question 9

What are 4 roles of RNA pol III?

Answer 9

• protein synthesis
• ribosomal component
• RNA splicing
• signal recognition particle for insertion of polypeptides into endoplasmic reticulum
• More unknown!

Question 10

What are the 4 commonalities in the 3 RNA polymerases?

Answer 10

• All exist in multimeric protein complexes
• Show significant similarity to bacterial subunits
• Determine function
• Elimination is lethal

Question 11

Explain CTD and what we see from it

Answer 11

It’s the staining of the carboxy terminal domain
- unphosphorylated stain green
- phosphorylated stain red
- Overlap is yellow

Chromosome puffs are where transcription is occurring (these are red areas)