RPA 6: Microbiology Flashcards
List 6 aseptic techniques
Wash hands with soap
Disinfect surfaces
Work close to a Bunsen flame
Open the Petri dish lid as little as possible
Use a sterile pipette/ flame the loop, bottle neck or spreader
Soak contaminated pipette tips in disinfectant
As an aseptic technique why should you wash hands with soap
To prevent contamination from bacteria on hands
What aseptic technique should you use to prevent contamination from bacteria on hands
Wash hands with soap
As an aseptic technique why should you disinfect surfaces
To kill bacteria on surfaces so contamination doesn’t occur
As an aseptic technique why should you work close to a Bunsen flame
To allow for upward convection currents to sterilise the air
As an aseptic technique why should you open the Petri dish lid as little as possible
To prevent bacteria in the dish getting out and unwanted bacteria in the air getting in and contaminating the plate
As an aseptic technique why should you use a sterile pipette/ flame the loop, bottle neck or spreader
To maintain a pure culture of bacteria
As an aseptic technique why should you soak contaminated pipette tips in disinfectant
To kill bacteria and prevent spreading outside the lab
For any aseptic technique that involves disinfecting/ using disinfectant, the reason for doing it is always to…
Kill the bacteria
(E.g disinfecting surfaces kills bacteria on the surfaces so that contamination doesn’t occur)
What aseptic technique is used to kill bacteria on surfaces so contamination doesn’t occur
Disinfect surfaces
What aseptic technique is used to allow for upward convection currents to sterilise the air
Work close to a Bunsen flame
What aseptic technique is used to prevent bacteria in the dish getting out and unwanted bacteria in the air getting in and contaminating the plate
Open the Petri dish lid as little as possible
What aseptic technique is used to maintain a pure culture of bacteria
Use a sterile pipette/ flame the loop, bottle neck or spreader
What aseptic technique is used to kill bacteria and prevent spreading outside the lab
Soak contaminated pipette tips in disinfectant
3 Variables to control when using filter paper discs containing different concentrations of the same disinfectant on the Agar plate
Same size/ thickness/ area/ diameter
Same material/ absorbency
Soaked in the disinfectant solution for the same time period
How does the antibiotic from the paper disc get into the agar
By diffusion
If a bacteria isn’t killed by the antibiotic it means that it’s …to it
Resistant
