RP6 Flashcards
Aseptic Techniques
1
Q
Aseptic Techniques
A
- Flame the neck of the container (Gets a pure culture of bacteria)
- Wear gloves (Prevents bacteria spreading)
- Lids on petri dish
(Prevents contamination of unwanted bacteria)
2
Q
Why used aseptic techniques
A
- Minimize risk of contamination
- Imprecise results
- Hazardous
3
Q
Why was agar boiled before pouring into the petri dish
A
Sterilize bacteria
4
Q
Why the Petri Dish stored upside down
A
Prevents condensation dripping on the surface of the agar
5
Q
Why was the plates not incubated at temperatures higher than 25oC
A
Mean it is closer to optimum temperature for the bacteria to cause human disease.
6
Q
Why was the original bacteria culture diluted
A
- Originally there would be high concentration of cells, and the counting of the original bacteria would be on inaccurate.
7
Q
Why was the same volume of bacteria transferred?
A
To enable comparison
8
Q
State some control variables
A
Same size of the paper discs
Same material
Soaked solutions for the same length of time
