RP1 - Microscopy Flashcards

1
Q

Describe how you would prepare a microscope slide for analysis

A

● Peel off epidermal layer using forceps
● Place sample on drop of water on microscope slide
● Add drops of iodine solution to sample
● Lower cover slip onto sample using mounting needle

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2
Q

Why is a thin sample of tissue required?

A

Allows light to pass through so internal structures can be observed

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3
Q

Why is water added to the slide before the sample is mounted?

A

Allows the sample to be suspended between the slide and the cover slip, and ensures the cover slip stays in place

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4
Q

Why is iodine solution added to stain the cells?

A

Iodine solution adds contrast - reacts with starch in plant cells and turns blue-black; allows internal structures (e.g. nucleus) to become more visible

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5
Q

Why is the cover slip lowered using a mounting needle?

A

Ensures that no air bubbles are trapped

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6
Q

How would you measure the length of a cell viewed under the microscope?

A

Measure using eyepiece graticule and calibrate with stage micrometer

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7
Q

How would you calculate the magnification of an image?

A

Magnification = observed cell length / actual cell length

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8
Q

What safety precautions should be taken during this experiment?

A

Wear safety goggles when handling iodine solution

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