RP 1 Flashcards
1
Q
give examples of variables that could affect the rate of an enzyme-controlled reaction
A
enzyme concentration
substrate concentration
temperature of solution
pH of solution
inhibitor concentration
2
Q
describe how temperature can be controlled in a reaction
A
use a thermostatically controlled water bath
monitor using a thermometer at regular intervals and add hot/cold water if temperature fluctuates
3
Q
describe how pH can be controlled
A
use a buffer solution
monitor using a Ph meter at regular intervals
4
Q
why were the enzyme and substrate solutions left in the water bath for 10 minutes before mixing
A
so solutions reach the temperature of the water bath
5
Q
describe a control experiment
A
use denatured anime’s
everything else the same as experiment
