Routine Urinalysis- Microscope Exam of Urine Flashcards

1
Q

Steps for proper speciman

A

Collection, Rapid transport to lab, and prompt examination (within 30 minutes-hour limit)

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2
Q

Volume of urine evaluated

A

12 mL (10-15mL range)

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3
Q

Centrifugation (speed and time)

A

400-450g for 5 minutes

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4
Q

Urine volume to resuspend sediment ratio

A

12:1

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5
Q

How many field of views for examination under microscope?

A

10-12

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6
Q

In what type of urine do you see RBCs crenate

A

Hypertonic urine

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7
Q

In what type of urine will you see RBC swell

A

Dilute or hypotonic urine (ghost RBC)

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8
Q

WBC in urine is called __________ and indicates an __________________ and the normal WBC/hpf is _________

A

Leukocyturia, infection (bacterial, non-bacterial), 0-8WBC/hpf

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9
Q

Non-granular WBC (lymphocytes) WILL or WILL NOT react with reagent strip creating a _______ ___________

A

WILL NOT react creating a FALSE NEGATIVE

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10
Q

What is the most common type of epithelial cell found in urine?

A

Squamous epithelial cell

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11
Q

Where do Squamous epithelial cells originate from?

A

superficial lining of urethra and vagina

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12
Q

Transitional cell originate where?

A

lining of renal pelvis, ureters, BLADDER, upper urethra

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13
Q

Increased numbers of Transitional epithelial cells may indicate what?

A

UTI, collection by catheterization, or malignancy

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14
Q

Renal tubular epithelial cells originate in lining of renal tubules and increased numbers indicate…

A

tubular necrosis

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15
Q

A clue cell is what type of cell with bacteria adhering to it? And presence of clue cells indicates what?

A

A squamous epithelial cell and may indicate bacterial vaginal infection.

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16
Q

Transitional epithelial cells are much larger than WBC because of abundant cytoplasm. What is the nucleus to cytoplasm ratio?

A

~1:5

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17
Q

Oval fat bodies (OFB) is what type of cell with absorbed fat?

A

Renal tubular epithelial

18
Q

The lab must see what using polarized microscopy to verify cell is OFB?

A

maltese cross

19
Q

OFB indicates what?

A

Pathology- associate with high protein in urine

20
Q

Large amounts of these may indicate improper collection technique

A

Squamous Epithelial cells

21
Q

Presence of casts reflect the health of the…

A

Renal tubules

22
Q

Normally, you can see few _________ casts or _______ casts

A

hyaline or granular

23
Q

Cast formation is increased by (4 things)

A

Acidity of urine, increased solute concentration, urine stasis, presence of plasma proteins

24
Q

The __________ cast is the most common cast seen in normal individuals (0-2 casts/lpf). Increased amounts seen with what?

A

Hyaline casts: seen with dehydration, fever, emotional stress, strenuous exercise

25
Q

RBC, WBC, epithelial, waxy, fatty, and broad casts are always

A

Pathologic

26
Q

True or false:

1) Casts are formed in the urine?
2) Acid pH and urine stasis are conductive for cast formation?
3) Casts are enumerated using high power objective?
4) Hyaline and RBC are not pathologic?

A

1) False
2) True
3) False
4) False

27
Q

If crystals are found in urine it is….

A

Pathologic

28
Q

All clinically significant crystals are found in (Acid urine/ alkaline urine)?

A

Acid urine

29
Q

Crystal formation is enhanced by (4 things)

A

Increased solute concentration, urine pH, urine stasis, and temperature (cooler temps)

30
Q

Normal acid pH crystals: (3)

A

Amorphous urates, uric acid, calcium oxalate

31
Q

The most frequently observed crystal in urine is

A

Calcium oxalate

32
Q

Normal Alkaline pH Crystals (3)

A

Amorphous phosphates, triple phosphate, ammonium biurate

33
Q

The most frequently observed crystal in alkaline urine is:

A

Triple phosphate crystals (coffin lid crystals)

34
Q

Pathologic crystals (acid, neutral pH): (5)

A

Cystine, tyrosine, leucine, cholesterol, bilirubin

35
Q

A positive ictotest will confirm what type of crystals

A

Bilirubin

36
Q

True or False:

1) Crystals are identified by shape and urine pH?
2) Uric acid crystals are found in acid urine and are shaped like a coffin lid?
3) Presence of ammonium biurate crystals indicate the urine is old?
4) Presence of bilirubin crystals indicate severe liver disease?

A

1- true
2- False
3- True
4- True

37
Q

Bacteria presence in urine may indicate what?

A

UTI or contamination

38
Q

You correlate nitrite reagent strip with what in urine?

A

Bacteria

39
Q

With bacteria in the urine, it is normally a ______ pH?

A

Alkaline

40
Q

This will not lyse with acetic acid or KOH

A

Yeast

41
Q

Are you have fun studying?

A

:) or :(