Routine Urinalysis- Microscope Exam of Urine Flashcards
Steps for proper speciman
Collection, Rapid transport to lab, and prompt examination (within 30 minutes-hour limit)
Volume of urine evaluated
12 mL (10-15mL range)
Centrifugation (speed and time)
400-450g for 5 minutes
Urine volume to resuspend sediment ratio
12:1
How many field of views for examination under microscope?
10-12
In what type of urine do you see RBCs crenate
Hypertonic urine
In what type of urine will you see RBC swell
Dilute or hypotonic urine (ghost RBC)
WBC in urine is called __________ and indicates an __________________ and the normal WBC/hpf is _________
Leukocyturia, infection (bacterial, non-bacterial), 0-8WBC/hpf
Non-granular WBC (lymphocytes) WILL or WILL NOT react with reagent strip creating a _______ ___________
WILL NOT react creating a FALSE NEGATIVE
What is the most common type of epithelial cell found in urine?
Squamous epithelial cell
Where do Squamous epithelial cells originate from?
superficial lining of urethra and vagina
Transitional cell originate where?
lining of renal pelvis, ureters, BLADDER, upper urethra
Increased numbers of Transitional epithelial cells may indicate what?
UTI, collection by catheterization, or malignancy
Renal tubular epithelial cells originate in lining of renal tubules and increased numbers indicate…
tubular necrosis
A clue cell is what type of cell with bacteria adhering to it? And presence of clue cells indicates what?
A squamous epithelial cell and may indicate bacterial vaginal infection.
Transitional epithelial cells are much larger than WBC because of abundant cytoplasm. What is the nucleus to cytoplasm ratio?
~1:5