Routine Media

Question 1

Blood Agar

Answer 1

Most pathogens

Question 2

Chocolate Agar (CHOC)

Answer 2

Haemophilus and Neisseria species

Question 3

Phenylethyl alcohol agar (PEA)

Answer 3

Selects for: Gram positive cocci and anaerobic gram neg rods

Question 4

What does CNA agar contain?

Answer 4

Colistin and nalidixic acid that suppresses gram negative growth

Question 5

CNA agar

Answer 5

Gram positive cocci, esp from wound and stool

Question 6

CHOC Aga

Question 7

What does Chocolate Agar (CHOC) contain?

Answer 7

Factors X (hemin) and V (NAD)

Question 8

Thayer Martin Agar

Answer 8

Neisseria gonorrhoeae and Neisseria meningitidis

Question 9

Thioglycolate broth

Answer 9

“back up” for anaerobes

Question 10

Lowenstein-Jensen Agar

Answer 10

Mycobacterium spp

Question 11

Middlebrook 7H10

Answer 11

Mycobacterium spp

Question 12

Petragnani Agar

Answer 12

Mycobacterium spp

Question 13

Brain Heart Infusion

Answer 13

Fastidious and non fastidious organisms

Question 14

EMB

Answer 14

Enteric GNR

Question 15

What does EMB contain?

Answer 15

Eosin and methylene blue to inhibit gram positive bacteria

E coli produces green metallic sheen
LF are green-black/purple
Non LF are colorless/light purple