RNA Splicing and Processing

Question 1

Lariat formation

Answer 1

Intron is cleaved at 5’ splice site and 5’ end is joined to 2’ position at an A in the branch site, intron then cleaved at 3’ site

Question 2

RNP

Answer 2

Ribonucleoprotein

Question 3

Splicing requires _____ and a ______

Answer 3

5’ and 3’ splice sites, branch site

Question 4

Intron definition

Answer 4

Splice sites defined by surrounding introns

Question 5

Transesterification

Answer 5

Exchanging ester for alcohol R groups

Question 6

Five snRNAs in splicing

Answer 6

U1, U2, U4, U5, U6

Question 7

Role of SR proteins in multicellular euks

Answer 7

Initiating formation of the commitment complex

Question 8

Nonsense-mediated mRNA decay (NMD)

Answer 8

Pathway that degrades an mRNA that has a nonsense mutation prior to last exon

Question 9

__ Complex, mature spliceosome

Answer 9

B1->B2

Question 10

__ Complex, commitment

Answer 10

E

Question 11

__ Complex, prespliceosome

Answer 11

A

Question 12

__ Complex, activated spliceosome

Answer 12

C

Question 13

Formation of commitment complex in which U1 is paired with 5’ splice site

Answer 13

E complex

Question 14

U2 addition to base pair with the branch site in presence of ATP

Answer 14

A complex

Question 15

Joining of U4/6 and U5 tri-snRNPs

Answer 15

B1 complex

Question 16

U1 and U4 release, formation of catalytic center where U6 base pairs with 5’ splice site and U2, U5 interacts with both exons

Answer 16

B2 complex

Question 17

Autosplicing/self-splicing

Answer 17

Autocatalytic splicing event, Group II introns from RNA

Question 18

First step of transesterification, 5’ splice site cleaved and lariat formed

Answer 18

C1 complex

Question 19

Second step of transesterification, 3’ splice site cleaved, exons ligated

Answer 19

C2 complex

Question 20

Splicing can occur ___ and ____ transcription

Answer 20

Before, after

Question 21

Sex determination in Drosophila involves series of ________

Answer 21

Alternative splicing events

Question 22

trans-splicing

Answer 22

short sequence (SL RNA) is spliced to 5’ ends of precursor mRNAs

Question 23

trans-splicing

Answer 23

short sequence (SL RNA) is spliced to 5’ ends of precursor mRNAs, set of cleavage and ligase reactions

Question 24

Ire1

Answer 24

Inner nuclear membrane protein, activation induced apoptosis when cell is overstressed by unfolded proteins

Question 25

5’ end of eukaryotic mRNA is ______

Answer 25

Capped by adding G to terminal base

Question 26

3’ ends of mRNAs generated by ____ and _____

Answer 26

Cleavage, polyadenylation

Question 27

Poly(A) tail importance

Answer 27

Controls mRNA stability and influences translation

Question 28

Histone mRNAs expression is _________

Answer 28

Replication dependent and regulated during cell cycle

Question 29

Are histone mRNAs polyadenylated?

Answer 29

No