Euk Transcription Regulation

Question 1

How is a gene turned on?

Answer 1

Activators and repressors, chromatin modeling and nucleosome recombination

Question 2

Eukaryotic gene expression controlled at level of _______

Answer 2

Initiation of transcription

Question 3

Insulators can stop spreading of ______ from one domain to another

Answer 3

Chromatin modifications

Question 4

_________ determine frequency of translation

Answer 4

Activators

Question 5

A positive transcription factor that makes contact, direct or indirect, with basal apparatus to activate transcription

Answer 5

True activator

Question 6

What is the default state of genes under positive control?

Answer 6

They cannot be expressed unless positive regulator is bound

Question 7

How is gene repression achieved?

Answer 7

Affecting chromatin structure or binding to and masking activators

Question 8

A protein that inhibits expression of a gene

Answer 8

Repressor

Question 9

What is the default state of genes under negative control?

Answer 9

They are expressed

Question 10

Architectural protein

Answer 10

A protein with the ability to alter DNA structure when bound to it

Question 11

________ is responsible for localizing a transcription-activating domain in the proximity of the _________

Answer 11

DNA-binding domain, basal apparatus

Question 12

How can activator without activating domain work?

Answer 12

Binds to coactivator with activating domain

Question 13

Activators classified according to __________?

Answer 13

Type of DNA-binding domain

Question 14

DNA-binding motifs examples

Answer 14

Zinc finger, homeodomain, helix-loop-helix, helix-turn-helix, leucine zipper

Question 15

Energy-dependent displacement or reorganization of nucleosomes that occurs in conjugation with activation of genes for transcription

Answer 15

Chromatin remodelling

Question 16

ATP-dependent chromatin remodeling complexes contain _______

Answer 16

Related ATPase catalytic subunit

Question 17

Remodeling complexes recruited to promoters by _________

Answer 17

Sequence specific activators (or repressors)

Question 18

Transcription activation often involves __________ at the promoter

Answer 18

Nucleosome displacement

Question 19

Major core histones contain conserved ___________

Answer 19

Histone-fold domain

Question 20

Nucleosomes precisely positioned at some __________ in a way for receptors to bind

Answer 20

Steroid-hormone response elements

Question 21

Histone acetylation associated with _________

Answer 21

Activation of gene expression

Question 22

Deacetylation associated with ______ of gene activity

Answer 22

Repression

Question 23

Repressor complex consists of (3):

Answer 23

DNA-binding subunit, corepressor, histone deacetylase

Question 24

Methylation of both DNA and specific sites on histones

Answer 24

Associated with inactive chromatin

Question 25

Remodeling complexes can facilitate ______ of acetyltransferase complexes

Answer 25

Binding

Question 26

NEAT1 expression dependent on _______

Answer 26

Heat Shock Transcription Factor 1 (HSF1)