RNA Flashcards
What is RNA?
- Polymer of a nucleotide
- Nucleotide formed of a Ribose, Nitrogenous base and a Phosphate group
- Single stranded polymer
What are the nitrogenous bases in RNA
Adenine
Uracil
Cytosine
Guanine
RNA has uracilinstead of thymine
What is the function of RNA?
To copy and transfer the genetic code from DNA in the nucleus to the ribosomes
- Some RNA is also combined with proteins to create ribosomes
What are the 3 types of RNA
mRNA
tRNA
rRNA
What is mRNA - messenger RNA
- A copy of a gene from DNA
- mRNA is short as it’s only the length of one gene so it can leave the nucleus
- It has a linear structure - single stranded
Where is mRNA created?
In the nucleus (transcription)
When DNA slightly unravels at the start of protein synthesis, a copy of the unraveled DNA is made in mRNA
- This is because DNA is too large to leave the nucleus
mRNA linear structure - codons
mRNA is single stranded and every 3 bases in the sequence codes for a specific amino acid
The 3 bases are called codons (mRNA triplets)
What is the function of mRNA?
- Leaves the nucleus to carry the copy of the genetic code of one gene to a ribosome in the cytoplasm
- mRNA is short lived ; it can code for the protein and then it gets degraded
Why can’t DNA leave the nucleus?
- It is too large and it can be exposed to enzymes which can hydrolyse the DNA or RNA polymers
What is the function of tRNA - transfer RNA?
- To attach to one of the 20 amino acids and **transfer that amino acid to the ribosome to create the polypeptide chain **
Where is tRNA found?
In the cytoplasm only
What is the shape of tRNA?
Single stranded but it folds to create a clover-leaf shape which is held in place by hydrogen bonds
- Hydrogen bonds between complementary base pairs
How do you determine which specific amino acid will attach to specific RNA molecules?
- Determined by 3 bases found on the tRNA which are complementary to the 3 bases on mRNA
What are anticodons?
Anticodons = 3 unattached bases
They are complementary to the codons on mRNA
Where is the amino acid binding site?
3 exposed bases at the top of the tRNA molecule are where the amino acid binds
mRNA vs tRNA
Feature | mRNA | tRNA
Shape | linear | Clover leaf
Bases | Codon | Anticodon
Formed | Nucleus | Cytoplasm
Involved | Transcription | Translation
Translation