rflp notes

Question 1

what’s a DNA fingerprint?

Answer 1

a pattern of bands made up of specific fragments form an individual’s DNA

Question 2

when is this technology used

Answer 2

for convicting criminals from bloodstains and determining the origin of diseases

Question 3

what is the method for a producing a DNA fingerprint

Answer 3

restriction fragment length polymorphism (RFLP) analysis

Question 4

Step 1:

Answer 4

the DNA sample is first cut into many fragments by a restriction enzyme

Question 5

Step 2:

Answer 5

The DNA fragments are then separated according to their size by gel electrophoresis

Question 6

what is gel electrophoresis

Answer 6

All DNA fragments are loaded at the top of the gel. Once electricity is run through the gel, the smaller DNA fragments will travel farther than larger DNA fragments

Question 7

Step 3:

Answer 7

Radioactive probes are added and bind to DNA fragments to help them become visible

Question 8

Step 4:

Answer 8

Photographic film is applied to take a picture of DNA fragments

Question 9

Step 5:

Answer 9

Photographic film is developed