Revision Session

Question 1

Fill in the blanks:
Cells movement in response to a chemical such as a GF is known as a ____. Cell migration in response to such signals can be measured in vitro using transwell inserts.
The cells are placed in the top well of a transwell insert in growth media with low serum which has low levels of ____.
Growth media with high serum is placed in the lower chamber.
Over time cancer cells will ____ through a ____ that separates the top chamber from the lower chamber. The presence of cells in the lower chamber can be ____ using a ____.

Answer 1

1. Chemotaxis
2. GFs
3. Migrate
4. Porous membrane
5. Measured
6. Stain

Question 2

Which of the following downstream signalling events involves hydrolysis of GTP?
- SH2 domain binding
- Ras activity
- MEK phosphorylation
- NFKappaB activation
- HIF dimerisation

Answer 2

Ras activity

Question 3

What are the receptors for the following growth factors:
- VEGF-C
- VEGF-A
- EGF
- HGF
- GCSF

Answer 3

• VEGF-C: Fli-4
• VEGF-A: KDR
• EGF: ErbB1
• HGF: c-Met
• GCSF: CD114

Question 4

What GFs are associated with these disease processes?:
- Metastasis
- Angiogenesis
- Lung cancer
- Liver metastasis
- Leukemia

Answer 4

• Metastasis: VEGF-C
• Angiogenesis: VEGF-A
• Lung cancer: EGF
• Liver metastasis: HGF
• Leukemia: GCSF

Question 5

Which of the following statements regarding radiotherapy for lung cancer are correct:
- Stereotactic ablative RT is rarely curative
- Continous hyperfractioned accelerated RT has ablative effects on tumours
- Squamous cell lung cancer is often more radiosensitive
- RT can be given pre- or post- operatively with similar effects on survival
- palliative RT improves pan in lung cancer in less than half of people

Answer 5

• Squamous cell lung cancer is often more radiosensitive
• RT can be given pre- or post- operatively with similar effects on survival

Question 6

Pertuzumab binds to which domain of HER2?

Answer 6

II