Revision Flashcards
Which AAs are hydrophobic (part of ER signal sequence)? (7)
- Valine
- Isoleucine
- Alanine
- Leucine
- Methionine
- Phenylalanine
- Tryptophan
Which hypothesis suggests that eukaryotic mitochondria evolved from early prokaryotes?
Endosymbiotic hypothesis
What supports the Endosymbiotic hypothesis?
- Mitochondria have their own circular genome
- their ribosomes resemble the 70S ones found in bacteria rather than the 80S found in eukaryotes
Which of the following diseases can be linked to the quality control function of the endoplasmic reticulum that retains misfolded proteins? There is more than one correct option. Answers: 1. alpha 1-antitrypsin deficiency 2. Buruli ulcer 3. Familial Hypercholesterolaemia 4. Cystic fibrosis 5. Alzheimer's disease
- alpha 1-antitrypsin deficiency
- cystic fibrosis
Two types of mutations located in the signal sequence of [x] are linked to Diabetes. In the one case the mutations perturb the interaction of the precursor protein with the [y] complex in the ER membrane and half of the newly made protein is not translocated. In the second case the protein is correctly translocated across the membrane but it does not [z] correctly inside the ER lumen.
x- insulin
y- sec61 translocon
z- fold
What has been identified as the molecular basis for chylomicron retention disease?
mutation to Sar1b
which part of the microtubule does EB1-GFP bind to?
+ end
where are lamins found?
the nucleus
where are neurofilaments found?
nerve cells
where are cytokeratins found?
epithelial cells
where are vementins found?
fibroblasts in connective tissue
true or false: The mitotic spindle is comprised of actin filaments and myosin filaments.
false
what’s the purpose of the hydrolysis of GTP to GDP carried out by tubulin molecules?
allows dynamic instability of microtubules
What would you predict would happen to the kinesin-coated silica beads if you were to add AMP-PNP (a nonhydrolyzable ATP analogue)?
The beads would stop moving as one molecule of ATP is hydrolyzed per step taken by kinesin
True or false: Microtubule-dependent motor proteins and microtubule polymerization and depolymerization are responsible for the organized movements of chromosomes during mitosis.
true
