Lecture 1: Cells and their components Flashcards

1
Q

What does cell theory state? (4)

A
  • cells are the fundamental units of life
  • all organisms are composed of cells
  • all cells form from pre-existing cells
  • all cells are surrounded by a plasma membrane
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2
Q

define unicellular organisms

A

a single cell that carries out all the functions of life

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3
Q

define multicellular organisms

A

made of many cells that are specialized for different functions

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4
Q

what 2 groups are included in prokaryotes?

A

Bacteria and Archaea

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5
Q

what 4 components to all prokaryotic cells have?

A

DNA, ribosomes, plasma membrane, cell wall

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6
Q

what 3 components do all eukaryotic cells have?

A
  • cytoskeleton
  • nucleus
  • complex internal membrane systems
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7
Q

what’s a important model cell system for prokaryote research?

A

E.Coli

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8
Q

What’s an important model cell system for lower eukaryote research?

A

Yeast

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9
Q

What’s an important model cell system for high eukaryote research?

A

Human tissue culture

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10
Q

what are 3 important model animals?

A

zebrafish, drosophila and Caenorhabditis elegans

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11
Q

What do Peroxisomes do?

A

break down fatty acids, alcohol and toxins

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12
Q

What disease can defects in peroxisome synthesis causse?

A

Zellweger syndrome

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13
Q

What is cytosol?

A
  • largest single compartment in the cell

- site of many fundamental cellular processes

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14
Q

What makes up the cytoplasm?

A

everything inside the plasma membrane outside the nucleus

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15
Q

What is the nucleus simply known as?

A

‘information store’

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16
Q

What is the nucleus surrounded by?

A

nuclear envelope

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17
Q

what does the nucleus contain?

A

cellular DNA and chromosomes

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18
Q

Where is the nucleolus found?

A

inside the nucleus

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19
Q

what does the nucleolus contain?

A

ribosomal RNA- to assemble ribosomal subunits

20
Q

what is the mitochondria’s main role?

A

energy generation

21
Q

what process occurs in the mitochondria?

A

oxidative phosphorylation to generate ATP

22
Q

What sort of membrane does the mitochondria have?

A

double membrane

23
Q

what’s the structure of the inner membrane of the mitochondria?

A

extensively folded (increase SA for cellular respiration)

24
Q

What’s the main role of the endoplasmic reticulum?

25
what's the structure of the endoplasmic reticulum?
irregular maze of interconnected spaces enclosed by a single membrane
26
What's the entry point to the secretory pathway?
ER
27
what does the ER make?
secretory and membrane proteins and lipids
28
What makes rough ER rough?
it has bound ribosomes
29
what is the sarcoplasmic reticulum?
ER derived Ca2+ store in muscle cells- for muscle contraction
30
what's the appearance of golgi?
flattened sacs/discs
31
what does the golgi do?
receives proteins and lipids as cargo from the ER- transits then to the plasma membrane
32
How do vesicles worth of material enter a cell?
endocytosis
33
how do vesicles of material leave a cell?
exocytosis
34
what makes up the endomembrane system?
nuclear envelope, ER and golgi
35
what's the purpose of microscopy?
to study tissues, cells and subcellular components
36
what's the purpose of centrifugation?
to isolate subcellular organelles
37
what's the purpose of chromatography?
purification of proteins and protein complexes
38
what's the purpose of gel electrophoresis and mass-spectrometry?
allow the analysis of macro-molecules like proteins and DNA
39
what is homogenisation?
the controlled rupture of plasma membrane
40
What are the 4 methods of homogenisation?
- break cells with high frequency e.g. ultrasound - use mild detergent to make holes in the plasma membrane - force cells through a small hole using high pressure - shear cells between a close-fitting rotating plunger and the thick walls of a glass vessels
41
what is differential centrifugation?
repeated centrifugation at progressively higher speeds will fractionate cell homogenates into their components
42
what does SDS stand for?
Sodium Dodecyl Sulphate
43
what does SDS do?
binds to proteins and unfolds them
44
what does PAGE stand for?
Poly Acrylamide Gel Electrophoresis
45
what sort of molecules is SDS- PAGE centrifugation used for?
protein
46
what's immunoblotting?
take SDS page and identify the specific protein using an antibody that recognises it