reviewer

Question 1

what is the greek word for color and write in chromatography?

Answer 1

-Chromatos
-Graphein

Question 2

phase that doesn’t move

Answer 2

Stationary Phase

Question 3

fluid entering the column

Answer 3

Eluent

Question 4

instrument employes

Answer 4

Chromatograph

Question 5

graph showing detector response as a function of time

Answer 5

Chromatogram

Question 6

process of passing the mobile phase through the column

Answer 6

Elution

Question 7

phase that flows through the column

Answer 7

Mobile Phase

Question 8

ability to separate molecules using various partitioning

Answer 8

Chromatography

Question 9

(mL/min)

Answer 9

Flow Rate

Question 10

(cm/min)

Answer 10

Linear Velocity

Question 11

specific interactions like a particular antibody protein

Answer 11

Affinity

Question 12

fluid exiting the column

Answer 12

Eluate

Question 13

attraction of ions with opposite charges

Answer 13

Ion Exchange

Question 14

based on the relative solubility of analyte in mobile and stationary

Answer 14

Partition

Question 15

state the ff. Rf values

Answer 15

b-carotene - 0.99
chlorophyll a - 0.30
chlorophyll b - 0.13
violaxanthin - 0.40
lutein - 0.68

Question 16

particles stick to the surface

Answer 16

Adsorption

Question 17

particles soak in the bulk of the other phase

Answer 17

Absorption

Question 18

state the leaf pigments

Answer 18

red - anthocyanins
orange - cartenoids
yellow - xanthophyll
green - chlorophyll

Question 19

give the formula for RF