Review Questions Flashcards
This type of protein group is symmetric and soluble in saline
Globular
What BUN/Creat ratio is associated with post renal azotemia?
10 – 20 : 1
This protein protects elastin in the lungs by inhibiting neutrophil elastase:
Alpha-1 antitrypsin
Which enzyme is found mostly in the intracellular fluid, specifically the cytoplasm and mitochondria?
AST
What enzyme is present in human saliva?
S amylase (salivary amylase)
For a technologist to successfully determine enzyme activity, which aspect of enzyme kinetics has to be overcome to determine activity?
First order
Which liver enzyme has the greatest specificity for damaged liver tissue?
ALT
This is an energy source utilized by muscles:
Creatine Phosphate
These type of proteins have > 40% carbohydrates attached:
Mucoproteins
This is a negative acute phase reactant that transports and stores iron:
Transferrin
Visible light at 700 nm appears as the color:
Red
What states: “The concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light”
Beers Law
This is the major excretory product of protein metabolism/catabolism:
Urea
Main protein constituent contributing to oncotic pressure:
Albumin
Urea is formed to neutralize the toxic effect of what waste product?
Ammonia
These types of proteins have 10-40% carbohydrates attached:
Glycoproteins
What enzyme is the most specific for acute pancreatitis?
Lipase
What BUN/Creat ratio is associated with pre-renal azotemia?
> 20:1
What liver marker is the most sensitive for alcoholism?
GGT
If the objective is to measure the enzyme activity, where on the initial velocity vs substrate concentration plot should the substrate concentration be fixed at?
Zero-Order Phase
Visible light at the 400 nm appears as the color
Violet
This is a transporter protein for T3, T4, and Vitamin-A:
Pre-albumin
Is Pre-Albumin a precursor to Albumin?
No, it just comes before albumin on electrophoresis
This is the product of the breakdown of purine bases:
Uric Acid
This type of protein group is asymmetric and water in-soluble:
Fibrous
This type of analytical method measures scattered light by placing a detector at a 90 degree angle:
Nephelometry
This kidney biomarker is not affected by diet:
Creatinine
The most common secondary structure of a protein is:
Alpha Helix
Enzyme historically used to assess prostate health before being replaced by PSA:
Acid Phosphatase
In the stomach, what breaks down proteins into peptides?
Pepsin
Another way to say “measurable range”:
Linear Range
This type of analytical method is prone to needing more frequent calibration due to background signals:
Chemiluminescence
This is caused by a deficiency in both protein and overall calories:
Marasmus
What is the one caused by deficiency in protein only?
Kwashiorkor
