Review Questions

Question 1

This type of protein group is symmetric and soluble in saline

Answer 1

Globular

Question 2

What BUN/Creat ratio is associated with post renal azotemia?

Answer 2

10 – 20 : 1

Question 3

This protein protects elastin in the lungs by inhibiting neutrophil elastase:

Answer 3

Alpha-1 antitrypsin

Question 4

Which enzyme is found mostly in the intracellular fluid, specifically the cytoplasm and mitochondria?

Answer 4

AST

Question 5

What enzyme is present in human saliva?

Answer 5

S amylase (salivary amylase)

Question 6

For a technologist to successfully determine enzyme activity, which aspect of enzyme kinetics has to be overcome to determine activity?

Answer 6

First order

Question 7

Which liver enzyme has the greatest specificity for damaged liver tissue?

Answer 7

ALT

Question 8

This is an energy source utilized by muscles:

Answer 8

Creatine Phosphate

Question 9

These type of proteins have > 40% carbohydrates attached:

Answer 9

Mucoproteins

Question 10

This is a negative acute phase reactant that transports and stores iron:

Answer 10

Transferrin

Question 11

Visible light at 700 nm appears as the color:

Answer 11

Red

Question 12

What states: “The concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light”

Answer 12

Beers Law

Question 13

This is the major excretory product of protein metabolism/catabolism:

Answer 13

Urea

Question 14

Main protein constituent contributing to oncotic pressure:

Answer 14

Albumin

Question 15

Urea is formed to neutralize the toxic effect of what waste product?

Answer 15

Ammonia

Question 16

These types of proteins have 10-40% carbohydrates attached:

Answer 16

Glycoproteins

Question 17

What enzyme is the most specific for acute pancreatitis?

Answer 17

Lipase

Question 18

What BUN/Creat ratio is associated with pre-renal azotemia?

Answer 18

> 20:1

Question 19

What liver marker is the most sensitive for alcoholism?

Answer 19

GGT

Question 20

If the objective is to measure the enzyme activity, where on the initial velocity vs substrate concentration plot should the substrate concentration be fixed at?

Answer 20

Zero-Order Phase

Question 21

Visible light at the 400 nm appears as the color

Answer 21

Violet

Question 22

This is a transporter protein for T3, T4, and Vitamin-A:

Answer 22

Pre-albumin

Question 23

Is Pre-Albumin a precursor to Albumin?

Answer 23

No, it just comes before albumin on electrophoresis

Question 24

This is the product of the breakdown of purine bases:

Answer 24

Uric Acid

Question 25

This type of protein group is asymmetric and water in-soluble:

Answer 25

Fibrous

Question 26

This type of analytical method measures scattered light by placing a detector at a 90 degree angle:

Answer 26

Nephelometry

Question 27

This kidney biomarker is not affected by diet:

Answer 27

Creatinine

Question 28

The most common secondary structure of a protein is:

Answer 28

Alpha Helix

Question 29

Enzyme historically used to assess prostate health before being replaced by PSA:

Answer 29

Acid Phosphatase

Question 30

In the stomach, what breaks down proteins into peptides?

Answer 30

Pepsin

Question 31

Another way to say “measurable range”:

Answer 31

Linear Range

Question 32

This type of analytical method is prone to needing more frequent calibration due to background signals:

Answer 32

Chemiluminescence

Question 33

This is caused by a deficiency in both protein and overall calories:

Answer 33

Marasmus

Question 34

What is the one caused by deficiency in protein only?

Answer 34

Kwashiorkor