review Q for connective, neuro & micro Flashcards
fixer in many silver techniques
extraneous reducer
oxidizer
fixer= sodium thiosulfate
extraneous reducer = formaldehyde
oxidizer= potassium permanganate
what is stained with the smallest dye molecule in trichrome method
RBC
which methods use parosanaline ( basic fuchsin )
Gomoris aldehyde fuchsin =yes
Luxol fast blue- PAS = Yes
Kinyoun= Yes
masson’s trichrome= NO
what is the mordant & oxidizer in Verhoeffs hematoxylin
ferric chloride ( differentiator & mordant) iodine ( mordant & oxidizes hematoxylin to hematien)
what is a control for Resorcin fuchsin staining
- stains elastic fibers
control = aorta
what is the function of bouins fluid in trichrome
mordant - makes staining more brilliant
Reticulin under certain conditions is PAS positive, it is also
Argyrophilic= requires an extraneous reducer
in silver impregnantion technique improperly cleaned glassware may result in
false positive
what is a sarcoma
what is carcinoma
sarcoma = a malignant neoplasm derived from connective tissue
carcinoma= a malignant neoplasm derived from epithelium ( car drives on top)
reticulin staining depends on the presence of
carbohydrates
Astrocytes can be demonstrated by which stainign technique
Cajal
section sfor myelin should be cut at what thickness
10-15micrometers
3- biopsies
5-6 = routine
6-8 = nissl substance
sections for Jones methenamine silver (JMS) should be cut at what thickness
basement membrane ( kidney) = 3 micrometers
which stains are for alziehmers diagnosis
Bielschowsky =
PAS= amyloid
Gallya =
Weil demonstrates myelin - doesnt help with diagnosis
demonstration of myelin in tissues depends on the presence of
lipids ( phospholipids)
Chromatolysis refers to loss of
nissl substance ( closest to nucleus)
Luxol fast blue has a structural formula closely related to
Alcian blue
Hydroquinine is used as a
reducer ( so is formaldehyde)
Acid fast stains may be falsely negative if this fixative is used
Alcoholic formalin ( alcohol can dissolve AFB)
Carnoy
in Kinyoun the target organism stain is what color
red
- kinyoun and zeil neilson are both dye methods that use carbol fuchsin to stain AFB
does PAS stain fungi
yes- least specific tho
what does Zeihl neilson stain
AFB ( so does kinyoun)
what deos warthin starry stain q
spirochetes
what does Giemsa stain
H.pylori
Carbol fuchsin methods are specific for
Acid fast Bacilli
- can be used to diagnose TB too ( along with kinyouin)
are spirochetes argarophilic or argentaphilic
they are are Argyrophilic
which method reuqires a lot of prep work & pre warmining
Warthin starry
which method would give a weak positive for Nocardia asteroides
Nocardia is partially acid fast
- KInyouin
what will bind Aldehyde fuchsin
Elastin & Schiff reagent
what type of tissue is examined for the presence of H.pylor
Gastric antrum
what stains Spirochetes
steiner & steiner
warthin starry
Dieterle
what does grocott stain
fungi
beta cells of the pancreas & pituitary gland stain deep purple with this stain
Gomoris aldehyde fuchsin
what is stained by Movat pentachrome
mucus, muscle, fibrin , elastin fibers, collagen
brown & hopps stains
Gram Neg
Brown & Bren stains
Gram pos
Lungs showing granulomatous lesions on H& E are generally stained with techniques to demonstrate
Fungus or acid fast bacilli
a PAS stained control for fungus is overatined & ID is difficult, this is a problem with
Periodic acid oxidiation ( not stong like chromic acid)
insoluble compnents that resist decoloriztion in kinyouin method are likely caused by
sections allowed to dry before differentiation
which pair of reagents is used as sensitizing reagents in metallic impregnantion
silver nitrate ( used in belchowsky ) and ferric ammonium sulfate ( used in gordon & sweets)
acid fast stain for leprosy ( fite stain)
xylene & peanut oil
what is the best stain to use as a screen fpr TB in s a suspected lung cancer stain
Auramine O ( fluorescnce for a quick screen- not a diagnosis)
a silver stained slide looks good, a ,onth later it has black precipitates all over this is due to
failure to use sodium thiosulfate
screen for Histoplasma capsulatum should use what stain
Grocott is the best method ( Gridley Giemsa & PAS are other fungi stains )
what causes sections to wash of slides during staining
alkalinity
- silver stains have alkaline silver solutions
too little silver development causes
yellow staining ( in silver staining goes from yellow to brown to black )
what AFB technique is best for small numbers of organisms
Auramine & rhodamine ( fluorescence )
a slide for fungus us overstained with PAS, hot to correct this
cut a new one and treat with diastase
if fungi are to be demonstrated a good counterstain for PAS is
light green
in the gridley procedure the aldeyhyde fuchsin stain will attach to
the schiff reagent
To differentiate Cryptococcus neoformans from other yeast like fungi, which of the following should be performed
Mayer mucicarmine
the difference between cryptococcus neoformans & other yeast like bacteria is that
cryptococcus has a capsule containing mucin
in the holzer technique, glial fibers are stained with
crystal violet
a method that can be used to demonstrated senile plaques is
Bielschowsky
Neuron cell bodies are found primairly in the
grey matter of the central nervous system
Spinal cord is a good control for which of the following
Weil
Glial fibers are stained blue by
Phosphotungstic acid -hematoxyling
macroscopic evaluation can be used to judge the following techniques
Weil
the PAS staine differs from Gridley technique in the
oxidizer used
Arifactual precipitate seen in Groccott stain may be the result of using
glassware that was not chemically cleaned
carbol-fuchsin methods are specific for
mycobacteria
the auromine-rhodamine technique will demonstrate
mycobcaterium tuberculosis
carbol fuchsin contains
basic fuchsin & phenol
stains for demonstration of spirochetes are based on their
Argarophilia
what is an example of physical staining
oil red O
fat is chemically fixed & maintained in tissue by
osmium tetraoxide
plasma cells can be demonstrated with
methyl green pyronin
when ferric ammonium sulfate is used in silver stain for reticulin, its function is
sensitizer
Mallory PTAH solution is ripened for immediate use by
potassium permanganate
when used in silver stain for reticulin, phosphomolybdic acid functions as the
oxidizer
