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Histology2 > review Q for connective, neuro & micro > Flashcards

review Q for connective, neuro & micro Flashcards

1
Q

fixer in many silver techniques
extraneous reducer
oxidizer

A

fixer= sodium thiosulfate

extraneous reducer = formaldehyde

oxidizer= potassium permanganate

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2
Q

what is stained with the smallest dye molecule in trichrome method

A

RBC

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3
Q

which methods use parosanaline ( basic fuchsin )

A

Gomoris aldehyde fuchsin =yes
Luxol fast blue- PAS = Yes
Kinyoun= Yes

masson’s trichrome= NO

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4
Q

what is the mordant & oxidizer in Verhoeffs hematoxylin

A 
ferric chloride ( differentiator & mordant) 
iodine ( mordant & oxidizes hematoxylin to hematien)
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5
Q

what is a control for Resorcin fuchsin staining

A
  • stains elastic fibers

control = aorta

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6
Q

what is the function of bouins fluid in trichrome

A

mordant - makes staining more brilliant

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7
Q

Reticulin under certain conditions is PAS positive, it is also

A

Argyrophilic= requires an extraneous reducer

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8
Q

in silver impregnantion technique improperly cleaned glassware may result in

A

false positive

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9
Q

what is a sarcoma

what is carcinoma

A

sarcoma = a malignant neoplasm derived from connective tissue

carcinoma= a malignant neoplasm derived from epithelium ( car drives on top)

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10
Q

reticulin staining depends on the presence of

A

carbohydrates

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11
Q

Astrocytes can be demonstrated by which stainign technique

A

Cajal

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12
Q

section sfor myelin should be cut at what thickness

A

10-15micrometers

3- biopsies
5-6 = routine
6-8 = nissl substance

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13
Q

sections for Jones methenamine silver (JMS) should be cut at what thickness

A

basement membrane ( kidney) = 3 micrometers

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14
Q

which stains are for alziehmers diagnosis

A

Bielschowsky =
PAS= amyloid
Gallya =

Weil demonstrates myelin - doesnt help with diagnosis

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15
Q

demonstration of myelin in tissues depends on the presence of

A

lipids ( phospholipids)

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16
Q

Chromatolysis refers to loss of

A

nissl substance ( closest to nucleus)

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17
Q

Luxol fast blue has a structural formula closely related to

A

Alcian blue

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18
Q

Hydroquinine is used as a

A

reducer ( so is formaldehyde)

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19
Q

Acid fast stains may be falsely negative if this fixative is used

A

Alcoholic formalin ( alcohol can dissolve AFB)

Carnoy

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20
Q

in Kinyoun the target organism stain is what color

A

red

- kinyoun and zeil neilson are both dye methods that use carbol fuchsin to stain AFB

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21
Q

does PAS stain fungi

A

yes- least specific tho

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22
Q

what does Zeihl neilson stain

A

AFB ( so does kinyoun)

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23
Q

what deos warthin starry stain q

A

spirochetes

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24
Q

what does Giemsa stain

A

H.pylori

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25
Q

Carbol fuchsin methods are specific for

A

Acid fast Bacilli

  • can be used to diagnose TB too ( along with kinyouin)
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26
Q

are spirochetes argarophilic or argentaphilic

A

they are are Argyrophilic

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27
Q

which method reuqires a lot of prep work & pre warmining

A

Warthin starry

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28
Q

which method would give a weak positive for Nocardia asteroides

A

Nocardia is partially acid fast

- KInyouin

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29
Q

what will bind Aldehyde fuchsin

A

Elastin & Schiff reagent

30
Q

what type of tissue is examined for the presence of H.pylor

A

Gastric antrum

31
Q

what stains Spirochetes

A

steiner & steiner
warthin starry
Dieterle

32
Q

what does grocott stain

A

fungi

33
Q

beta cells of the pancreas & pituitary gland stain deep purple with this stain

A

Gomoris aldehyde fuchsin

34
Q

what is stained by Movat pentachrome

A

mucus, muscle, fibrin , elastin fibers, collagen

35
Q

brown & hopps stains

A

Gram Neg

36
Q

Brown & Bren stains

A

Gram pos

37
Q

Lungs showing granulomatous lesions on H& E are generally stained with techniques to demonstrate

A

Fungus or acid fast bacilli

38
Q

a PAS stained control for fungus is overatined & ID is difficult, this is a problem with

A

Periodic acid oxidiation ( not stong like chromic acid)

39
Q

insoluble compnents that resist decoloriztion in kinyouin method are likely caused by

A

sections allowed to dry before differentiation

40
Q

which pair of reagents is used as sensitizing reagents in metallic impregnantion

A 
silver nitrate  ( used in belchowsky ) 
and ferric ammonium sulfate ( used in gordon & sweets)
41
Q

acid fast stain for leprosy ( fite stain)

A

xylene & peanut oil

42
Q

what is the best stain to use as a screen fpr TB in s a suspected lung cancer stain

A

Auramine O ( fluorescnce for a quick screen- not a diagnosis)

43
Q

a silver stained slide looks good, a ,onth later it has black precipitates all over this is due to

A

failure to use sodium thiosulfate

44
Q

screen for Histoplasma capsulatum should use what stain

A

Grocott is the best method ( Gridley Giemsa & PAS are other fungi stains )

45
Q

what causes sections to wash of slides during staining

A

alkalinity

- silver stains have alkaline silver solutions

46
Q

too little silver development causes

A

yellow staining ( in silver staining goes from yellow to brown to black )

47
Q

what AFB technique is best for small numbers of organisms

A

Auramine & rhodamine ( fluorescence )

48
Q

a slide for fungus us overstained with PAS, hot to correct this

A

cut a new one and treat with diastase

49
Q

if fungi are to be demonstrated a good counterstain for PAS is

A

light green

50
Q

in the gridley procedure the aldeyhyde fuchsin stain will attach to

A

the schiff reagent

51
Q

To differentiate Cryptococcus neoformans from other yeast like fungi, which of the following should be performed

A

Mayer mucicarmine

52
Q

the difference between cryptococcus neoformans & other yeast like bacteria is that

A

cryptococcus has a capsule containing mucin

53
Q

in the holzer technique, glial fibers are stained with

A

crystal violet

54
Q

a method that can be used to demonstrated senile plaques is

A

Bielschowsky

55
Q

Neuron cell bodies are found primairly in the

A

grey matter of the central nervous system

56
Q

Spinal cord is a good control for which of the following

A

Weil

57
Q

Glial fibers are stained blue by

A

Phosphotungstic acid -hematoxyling

58
Q

macroscopic evaluation can be used to judge the following techniques

A

Weil

59
Q

the PAS staine differs from Gridley technique in the

A

oxidizer used

60
Q

Arifactual precipitate seen in Groccott stain may be the result of using

A

glassware that was not chemically cleaned

61
Q

carbol-fuchsin methods are specific for

A

mycobacteria

62
Q

the auromine-rhodamine technique will demonstrate

A

mycobcaterium tuberculosis

63
Q

carbol fuchsin contains

A

basic fuchsin & phenol

64
Q

stains for demonstration of spirochetes are based on their

A

Argarophilia

65
Q

what is an example of physical staining

A

oil red O

66
Q

fat is chemically fixed & maintained in tissue by

A

osmium tetraoxide

67
Q

plasma cells can be demonstrated with

A

methyl green pyronin

68
Q

when ferric ammonium sulfate is used in silver stain for reticulin, its function is

A

sensitizer

69
Q

Mallory PTAH solution is ripened for immediate use by

A

potassium permanganate

70
Q

when used in silver stain for reticulin, phosphomolybdic acid functions as the

A

oxidizer

Histology2 (14 decks)

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