Hematoxylin & Eosin

Question 1

Nuclear dye

Answer 1

• Hemstoxylin is most widely used
• H&E is the ‘routine’ or oversight stain
• Only suitable substitute is Celestine Blue

Question 2

” special staining methods

Answer 2

• dye staining
• elective or selective solubility with lysochromes ( fat stains )
• true histochemical reactions (ex. iron)
• Metallic impregnantion ( aka silver stains)
• immunohisrochemistry
• Enzymatic histochemistry

Question 3

Hematoxylin

Answer 3

the most used stain in histology
- the routine/ oversight stain
- substitute = Celestine Blue ( which must be mordanted with iron)\
- extraction of logwood tree, campeche mexico
- not a dye until oxidized
- hematein is the dye

Question 4

oxidation of hematoxylin

Answer 4

natural or artificial
- natural through air & sunlight
- artificial involves chemicals