Reverse-Transcriptase (RT) PCR Flashcards
Better understand the mechanism
1
Q
What is RT-PCR?
A
When an RNA strand is reverse transcribed into its DNA complement using the reverse transcriptase (such as SuperScript).
The most sensitive technique for detecting the presence of rotavirus.
2
Q
What is transcription?
A
When DNA is used as a template to synthesize RNA.
3
Q
What is reverse transcription?
A
When a series of nucleotides is copied from an RNA template to synthesise DNA.
4
Q
What is reverse transcriptase?
A
An RNA-dependent DNA polymerase.
5
Q
What is a rotavirus?
A
Any group of RNA viruses.
