reverse Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

PEPCK

A
  • Binding of GR signal leads to chromatin remodeling (turn on gene by displacement of histories by acctyylation of histone tails).
  • In the PEPCK example you saw a simple case where the binding of an activator (GR protein) helps recruitment of a chromatin remodelling complex. This is an example of cooperative binding and is typical in eukaryotes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Combinatorial control

A
  • Transcription factors can be shared between target genes to be regulated. This is because they act in combination with others to differentially regulate the expression of the targets.
  • The activity of a SOX protein is dependent upon the identity of its partner and the context of the DNA sequence to which it binds.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

SOX partners

A
  • Bind to partners (other sox or other proteins. ).
  • Flexibility in DNA motif recognition.
  • spacing between DNA binding motifs
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Epigenetic inheritance

A

Changes in cellular or physiological state that are not caused by changes in DNA sequence
These non-DNA sequence changes affect gene expression.
•How?
–Acetylation
–Deacetylation
–Methylation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Position effect silencing

A

Genes can be on or off depending on their position in the genome and such position can change due to mutations. chromosome rearrangements can:

  1. can place genes in geterchromatoc regions (eg drosophila age variation)
  2. place genes user direct regulaoty elements.
    - -drosophila and tissue in thorax.
    - -follicular lymphomas
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Methylation and imprinting

A

(sex-specific differences)

Notice that imprinting means only the Igf2 allele inherited from the father is actively expressed in the embryo

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Methylation and gene expression

A

1) Methylation affects regulation by modifying chromatin structure
2) Methylationof C (CG islands) in genes can directly silence genes by restricting TFs binding. Similarly lack of methylation promotes gene expression

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Acetylation histones and chromatin remodeling

A

facilitates displacement of histoneoctamerexposes DNA to transcription factors.xpression

How well did you know this?
1
Not at all
2
3
4
5
Perfectly