restriction enzymes/maps and recontaminate DNA,

Question 1

what is a nucleas and that are the 2 kind

Answer 1

enzyme that breaks down nucleic acid

DNase, and RNase

Question 2

DNase exonuclease

Answer 2

chews up DNA from the ends

Question 3

DNase endonuclease

Answer 3

cuts DNA in the middle of the molecule can be non specific or specific

Question 4

restriction enzymes are

Answer 4

endonuclease that cut DNA at specific sequence

Question 5

EcoR1 recognizes _______ and leaves a _bp sticky end

Answer 5

“GAATTC” , 6

Question 6

what enzymes leave a blunt end

Answer 6

Alu1,Haelll,Sma1

Question 7

Alu recognizing _ bp, and Not1 recognizing _ bp

Answer 7

4,8

Question 8

if there is 5 sites of a GAATC that EcoR1 cuts how many peices will be cut

Answer 8

5 pieces

Question 9

why is DNA negatively charges

Answer 9

the phosphate groups PO4-

Question 10

in gel electrophoresis

Answer 10

short dna molecules will move faster through the gel than large strands

Question 11

what is dna in electrolysis stained with

Answer 11

ethiduim bromide

Question 12

what is recombination dna

Answer 12

DNA that has been formed artificially by combining constituents from different organisms.

Question 13

what are 3 things needed to make recombinate dna

Answer 13

purified DNA, vector, dna ligase

Question 14

what are useful properties of restriction enzymes

Answer 14

cut DNA at specific sites, can produce sticky ends

Question 15

what is a cloning vector

Answer 15

small DNA molecule that can replicate in a host bacterial cell (must have a origin or replication)

Question 16

what are some examples of cloning vectors

Answer 16

plasmid or phage chrosome

Question 17

what should a suitable cloning vector have

Answer 17

1) should carry a selectable genetic markers
2) have one or more unique restriction sites - squenece that is cute by a restr. enzyme only once in the vector
3) should be a way to easily distinguish recombinate DNA

Question 18

what are the 3 possiblites when cut DNA and vectors with sticky ends are mixed

Answer 18

DNA fragment cirlce forms
the vector cirles re-forms
recombinant DNA forms

Question 19

add ____ to the recombin. mixture to covalently join ends

Answer 19

ligase

Question 20

what help you determine whether the bacteria took up vector circle or recombinat DNA

Answer 20

the bacteria is lacZ negative and the plasmid contains the lacZ gene with the coding region for beta (B-galactosidase)

Question 21

how does X-gal work

Answer 21

when colonies are grown in X-gal the ones that produce B-galactosidase will turn blue which means that it is the vector DNA, the recomb. Dna will be white

Question 22

what is genomic DNA

Answer 22

all of an organisms genes inclung introns and junk da

Question 23

genomic library

Answer 23

collection of 100’s of deff. recomb. DNA molecules made by ligating genomic DNA fragments into cloning vector

Question 24

cDNA library

Answer 24

is used for protein coding and is only comprised or coding regions

Question 25

cDNA is made from

Answer 25

mRNA and using reverse transcritase to copy mRNA to DNA copy