DNA forensics and PCR Flashcards
non coding regions have _____ variability between individuals
a lot of
why do non coding regions have lots or varability
silent mutations do not effect the phenotype
DNA sequence polymorphisms
differences in DNA sequences that exist between individuals in a population
restriction fragment length polymorphic RFLP
Diff. in restriction enzyme digestion patterns that exist between indiv. b/c of diff. in DNA sequences
northen blot is
method to identify RNA on a gel by hybridizing to coloned DNA probe
southern blot is
method to identify DNA fragments on a gel by hybridizing to cloned DNA probe
western blots is
method to identify protein on a gel by probing with an antibody specific for the protein of interest.
the northen blot and southern blot techniques are
nearly idenitcal
DNA firger prints are ___________ that use probes as regions of the genome that are _________
southern blots, highly variable
VNTR’s are
variable number tandem repeats simple sequences repeated many times in a row
in practice you would use multiple _______ for diff. VNTR’s so it will give______ patterns for each individual
probes, complex
what is the disadvantage of southern blot/RLFP analysis
need lots of DNA, , and it will not work with old/degraded dna
what are the components or a pcr
- dna template 2. 4 dNTP’s 3, buffer 4. primers 5. dna polymerase (taq)
taq is used b/c
it is very hear resistant
how many times does this process
25-40 cylces
