restriction enzymes Flashcards
1
Q
uses
A
cuts up DNA for better study
2
Q
how does it work
A
each restriction enzyme cuts at a specific DNA sequence -
3
Q
applications
A
Gel electrophoresis
-cut up DNA of invading bacteria, viruses…
4
Q
blunt ends
A
cuts straight across both strands of DNA
5
Q
what is needed to stick bluntends back togethert
A
ligase
6
Q
Sticky ends
A
staggared cut that leaves tail of free DNA on either side - dont need anythiong to reattahch
